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. 1982 Aug;79(15):4594-8.
doi: 10.1073/pnas.79.15.4594.

Site-directed psoralen crosslinking of DNA

Site-directed psoralen crosslinking of DNA

W A Saffran et al. Proc Natl Acad Sci U S A. 1982 Aug.

Abstract

A technique has been developed for placing site-specific crosslinks into DNA by using a psoralen derivative containing a sulfhydryl group. Plasmid pBR322 was nicked at the unique BamHI restriction site, and mercurated pyrimidines were introduced by nick-translation. Then the psoralen was specifically directed to this site in the dark through a Hg-S linkage prior to irradiation to form a crosslink. The location of interstrand cross-links was examined by electron microscopy of Pst I-cut molecules and found to be at or near the BamHI site.

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