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. 1982 Mar 25;10(6):1963-79.
doi: 10.1093/nar/10.6.1963.

Cloning and determination of the transcription termination site of ribosomal RNA gene of the mouse

R Kominami, Y Mishima, Y Urano, M Sakai, M Muramatsu

Cloning and determination of the transcription termination site of ribosomal RNA gene of the mouse

R Kominami et al. Nucleic Acids Res. .

Abstract

A Eco RI 6.6 kb DNA fragment containing the 3'-end of 28S ribosomal RNA gene of the mouse was detected by Southern blot hybridization, and cloned in a lambda-phage vector. The site of transcription termination and the processed 3'-end of 28S RNA were determined on the cloned fragment and the surrounding nucleotide sequence determined. The 3'-terminal nucleotides of mouse 28S RNA are similar to those of yeast, Drosophila and Xenopus although the homology was lost drastically beyond the 3'-end of 28S RNA. 45S precursor RNA terminated at 30 nucleotides downstream from the 3'-end of 28S RNA gene. A structure of a dyad symmetry with a loop was found immediately prior to the termination site of 45S RNA. The rDNA termination site thus shares some common features with termination sites recognized by other RNA polymerases.

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References

    1. Eur J Biochem. 1973 Jul 2;36(1):32-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4579-83 - PubMed
    1. J Mol Biol. 1974 Oct 25;89(2):397-407 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 - PubMed
    1. J Mol Biol. 1975 May 5;94(1):51-69 - PubMed

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