Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 Apr;38(1):50-8.
doi: 10.1128/JVI.38.1.50-58.1981.

Cloning of herpes simplex virus type 1 sequences representing the whole genome

A L Goldin, R M Sandri-Goldin, M Levine, J C Glorioso

Cloning of herpes simplex virus type 1 sequences representing the whole genome

A L Goldin et al. J Virol. 1981 Apr.

Abstract

Sequences representative of the whole genome of herpes simplex virus type 1 (HSV-1) strain KOS were cloned in the plasmid vector pBR325 in the form of EcoRI-generated DNA fragments. The cloned fragments were identified by digestion of the chimeric plasmid DNA with restriction enzymes EcoRI or EcoRI and BglII followed by comparison of their electrophoretic mobilities in agarose gels with that of similarly digested HSV-1 virion DNA. The cloned fragments showed the same migration patterns as the corresponding fragments from restricted virion DNA, indicating that no major insertions or deletions were present. The presence of HSV-1 sequences in the chimeric plasmids was confirmed by hybridization of plasmid DNA to HSV-1 virion DNA. Additionally, some of the cloned fragments were shown to be biologicaly active in that they efficiently rescued three HSV-1 temperature-sensitive mutants in cotransfection marker rescue experiments.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 - PubMed
    1. J Virol. 1980 Mar;33(3):1221-4 - PubMed
    1. Biochemistry. 1970 Oct 27;9(22):4428-40 - PubMed
    1. Virology. 1980 Apr 15;102(1):172-89 - PubMed
    1. Gene. 1980 May;9(3-4):287-305 - PubMed

Publication types

LinkOut - more resources