Construction of an adenovirus-SV40 recombinant producing SV40 T antigen from an adenovirus late promoter
- PMID: 6263482
- DOI: 10.1016/0092-8674(81)90509-2
Construction of an adenovirus-SV40 recombinant producing SV40 T antigen from an adenovirus late promoter
Abstract
An adenovirus-SV40 recombinant has been constructed in which the SV40 early region, joined to a copy of the adenovirus major late promoter, was substituted for a small portion of the adenovirus late region and all of early region 3. The inserted sequence functions as a separate transcriptional unit, using the ectopic adenovirus promoter, SV40 splice sites and SV40 polyadenylation site. The RNAs produced lack both the second and third members of the tripartite leader normally found on adenovirus late RNA, yet are translationally active, directing the synthesis of substantial amounts of SV40 T antigen. The recombinant may serve as a model vehicle for the abundant expression of other eucaryotic genes inserted into the adenovirus genome.
Similar articles
-
Translational control of SV40 T antigen expressed from the adenovirus late promoter.Cell. 1983 Jun;33(2):455-64. doi: 10.1016/0092-8674(83)90427-0. Cell. 1983. PMID: 6305512
-
Expression of SV40 T antigen under control of adenovirus promoters.Cell. 1981 Mar;23(3):825-36. doi: 10.1016/0092-8674(81)90447-5. Cell. 1981. PMID: 7226231
-
Deletions covering the putative promoter region of early mRNAs of simian virus 40 do not abolish T-antigen expression.Proc Natl Acad Sci U S A. 1980 Jul;77(7):3865-9. doi: 10.1073/pnas.77.7.3865. Proc Natl Acad Sci U S A. 1980. PMID: 6253995 Free PMC article.
-
Regulation of viral transcription and DNA replication by the SV40 large T antigen.Curr Top Microbiol Immunol. 1981;93:5-24. doi: 10.1007/978-3-642-68123-3_2. Curr Top Microbiol Immunol. 1981. PMID: 6269805 Review. No abstract available.
-
Functions of T antigens of SV40 and polyomavirus.Biochim Biophys Acta. 1981 Aug 31;651(1):1-24. doi: 10.1016/0304-419x(81)90002-0. Biochim Biophys Acta. 1981. PMID: 6169368 Review. No abstract available.
Cited by
-
Identification of a previously undescribed divergent virus from the Flaviviridae family in an outbreak of equine serum hepatitis.Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):E1407-15. doi: 10.1073/pnas.1219217110. Epub 2013 Mar 18. Proc Natl Acad Sci U S A. 2013. PMID: 23509292 Free PMC article.
-
A helper-dependent adenovirus vector system: removal of helper virus by Cre-mediated excision of the viral packaging signal.Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13565-70. doi: 10.1073/pnas.93.24.13565. Proc Natl Acad Sci U S A. 1996. PMID: 8942974 Free PMC article.
-
Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.Nucleic Acids Res. 1981 Oct 24;9(20):5233-52. doi: 10.1093/nar/9.20.5233. Nucleic Acids Res. 1981. PMID: 7301588 Free PMC article.
-
Transformation-deficient adenovirus mutant defective in expression of region 1A but not region 1B.J Virol. 1982 Apr;42(1):106-13. doi: 10.1128/JVI.42.1.106-113.1982. J Virol. 1982. PMID: 7086960 Free PMC article.
-
Shuffling adenovirus promoters: a viral recombinant with early region 1A under late transcriptional control.EMBO J. 1983;2(6):845-51. doi: 10.1002/j.1460-2075.1983.tb01512.x. EMBO J. 1983. PMID: 6641719 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Medical