Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 Jan;37(1):395-410.
doi: 10.1128/JVI.37.1.395-410.1981.

Evolutionary variants of simian virus 40 which are impaired in early lytic functions but transform nonpermissive cells

Evolutionary variants of simian virus 40 which are impaired in early lytic functions but transform nonpermissive cells

C J Sol et al. J Virol. 1981 Jan.

Abstract

From an undiluted passaged virus stock, two size classes of defective simian virus 40 (SV40) DNA were isolated from which two evolutionary variants were cloned. By means of restriction enzyme and heteroduplex analysis, physical maps of the mutants have been constructed. Both mutants contained the region of SV40 DNA coding for the early proteins plus some adjacent sequences (the region from 0.120 to 0.685 map unit, clockwise, on the standard SV40 DNA map). Furthermore, each mutant contained, in the form of two inverted repeats, four times the sequences from the region 0.625 to 0.685 map unit, clockwise. Some biological properties of the mutant DNA were examined, and we found that the mutant DNA (i) has, as compared with SV40 DNA, an impaired ability to induce T antigen in permissive and nonpermissive cells; (ii) does not complement a thermosensitive A mutant of SV40; (iii) replicates very inefficiently without a helper; and (iv), as an apparent contradiction, transforms nonpermissive baby rat kidney cells as well as SV40 DNA. A hypothetical mechanism for the expression of the mutant DNA that might explain the observed biological properties is presented.

PubMed Disclaimer

Similar articles

Cited by

References

    1. J Mol Biol. 1967 Jun 14;26(2):365-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1967 May;57(5):1514-21 - PubMed
    1. J Natl Cancer Inst. 1968 Aug;41(2):351-7 - PubMed
    1. Nat New Biol. 1971 Jul 21;232(29):73-6 - PubMed
    1. J Mol Biol. 1971 Sep 28;60(3):473-84 - PubMed

Publication types

LinkOut - more resources