Mouse immunoglobulin genes: a bacterial plasmid containing the entire coding sequence for a pre-gamma 2a heavy chain
- PMID: 6253932
- PMCID: PMC323988
- DOI: 10.1093/nar/8.6.1231
Mouse immunoglobulin genes: a bacterial plasmid containing the entire coding sequence for a pre-gamma 2a heavy chain
Abstract
A DNA sequence complementary to the entire coding part of a mouse gamma 2a immunoglobulin heavy chain mRNA isolated from a myeloma producing a levan binding protein (UPC 10), has been cloned in the PstI site of pBR 322. Transformants containing sequences complementary to purified gamma 2a heavy chain mRNA were selected. One transformant, pG2a-10-21, containing a 1750 nucleotide insert, has been characterized by hybrid-arrested translation and purification of gamma 2a heavy chain mRNA on DNA-DBM cellulose filters. Restriction enzyme analysis and partial sequencing demonstrate that the pG2a-10-21 contains the complete structural sequence for the gamma 2a heavy chain and predicts the sequence of a 18 amino acid hydrophobic amino terminal extra piece segment.
Similar articles
-
Immunoglobulin gamma 1 heavy chain gene: structural gene sequences cloned in a bacterial plasmid.Gene. 1980 Apr;9(1-2):87-97. doi: 10.1016/0378-1119(80)90168-7. Gene. 1980. PMID: 6769752
-
Molecular cloning of rabbit gamma heavy chain mRNA.Nucleic Acids Res. 1982 Mar 11;10(5):1535-45. doi: 10.1093/nar/10.5.1535. Nucleic Acids Res. 1982. PMID: 6280149 Free PMC article.
-
Structure of immunoglobulin gamma 2b heavy chain gene cloned from mouse embryo gene library.Nucleic Acids Res. 1980 Feb 11;8(3):453-66. doi: 10.1093/nar/8.3.453. Nucleic Acids Res. 1980. PMID: 6255443 Free PMC article.
-
Structure of the constant and 3' untranslated regions of the murine Balb/c gamma 2a heavy chain messenger RNA.Nucleic Acids Res. 1980 Jul 25;8(14):3143-55. doi: 10.1093/nar/8.14.3143. Nucleic Acids Res. 1980. PMID: 6777755 Free PMC article.
-
Human antibody genes: V gene variability and CH gene switching strategies.Immunol Rev. 1981;59:69-91. doi: 10.1111/j.1600-065x.1981.tb00456.x. Immunol Rev. 1981. PMID: 6273296 Review. No abstract available.
Cited by
-
Expression of the genes coding for the skeletal muscle and cardiac actions in the heart.Nucleic Acids Res. 1984 Jan 25;12(2):1087-100. doi: 10.1093/nar/12.2.1087. Nucleic Acids Res. 1984. PMID: 6546444 Free PMC article.
-
Expression of several amplified genes in an adenylate-deaminase overproducing variant of Chinese hamster fibroblasts.EMBO J. 1984 Dec 20;3(13):3123-7. doi: 10.1002/j.1460-2075.1984.tb02268.x. EMBO J. 1984. PMID: 6526013 Free PMC article.
-
cDNA sequences of Torpedo marmorata acetylcholinesterase: primary structure of the precursor of a catalytic subunit; existence of multiple 5'-untranslated regions.EMBO J. 1987 Jul;6(7):1865-73. doi: 10.1002/j.1460-2075.1987.tb02445.x. EMBO J. 1987. PMID: 2820709 Free PMC article.
-
Molecular cloning and nucleotide sequence of a human renin cDNA fragment.Nucleic Acids Res. 1983 Oct 25;11(20):7181-90. doi: 10.1093/nar/11.20.7181. Nucleic Acids Res. 1983. PMID: 6138751 Free PMC article.
-
Identification of a cDNA clone coding for the acetylcholine binding subunit of Torpedo marmorata acetylcholine receptor.EMBO J. 1982;1(6):713-7. doi: 10.1002/j.1460-2075.1982.tb01235.x. EMBO J. 1982. PMID: 6897919 Free PMC article.
References
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
