doi: 10.1099/0022-1317-48-1-213.
The polypeptide composition of isolated surface projections of avian infectious bronchitis virus
- PMID: 6247435
- DOI: 10.1099/0022-1317-48-1-213
Item in Clipboard
The polypeptide composition of isolated surface projections of avian infectious bronchitis virus
J Gen Virol.
1980 May.
Abstract
Disruption of avian infectious bronchitis virus (IBV) particles with 4% Triton X-100 and 1.0 M-KCl and centrifugation through a sucrose gradient containing 0.1% Triton X-100 and 1.0 M-KCl enabled separation of the petal-shaped surface projections. By negative-contrast electron microscopy the separated projections appeared mainly as rosettes containing 3 to 12 projections radiating from a central core, although single projections and rosettes containing up to 16 projections were seen. SDS-PAGE of these preparations revealed two polypeptides of 86,000 and 66,000 mol. wt. The larger polypeptide was glycosylated.
Similar articles
-
The polypeptides of infectious bronchitis virus (IBV-41 strain).J Gen Virol. 1980 Feb;46(2):349-61. doi: 10.1099/0022-1317-46-2-349. J Gen Virol. 1980. PMID: 6247427
-
Coronavirus IBV: further evidence that the surface projections are associated with two glycopolypeptides.J Gen Virol. 1983 Aug;64 (Pt 8):1787-91. doi: 10.1099/0022-1317-64-8-1787. J Gen Virol. 1983. PMID: 6308136
-
The purification and polypeptide composition of avian infectious bronchitis virus.Microbios. 1977;18(72):87-98. Microbios. 1977. PMID: 205761
-
Two particle types of avian infectious bronchitis virus.J Gen Virol. 1980 Apr;47(2):365-72. doi: 10.1099/0022-1317-47-2-365. J Gen Virol. 1980. PMID: 6245175
-
Structural polypeptides of coronavirus IBV.J Gen Virol. 1981 Mar;53(Pt 1):93-103. doi: 10.1099/0022-1317-53-1-93. J Gen Virol. 1981. PMID: 6268743
Cited by
-
Characterisation of rubella virus hemagglutinin rosettes.J Virol Methods. 1981 Mar;2(4):195-201. doi: 10.1016/0166-0934(81)90009-4. J Virol Methods. 1981. PMID: 7228976 Free PMC article.
-
The molecular biology of coronaviruses.Adv Virus Res. 1983;28:35-112. doi: 10.1016/s0065-3527(08)60721-6. Adv Virus Res. 1983. PMID: 6362367 Free PMC article. Review.
-
Antigenicity of mouse hepatitis virus strain 3 subcomponents in C57 strain mice.Arch Virol. 1981;69(1):33-41. doi: 10.1007/BF01315263. Arch Virol. 1981. PMID: 6271096 Free PMC article.
-
Antibody to virus components in volunteers experimentally infected with human coronavirus 229E group viruses.Infect Immun. 1981 Mar;31(3):845-9. doi: 10.1128/iai.31.3.845-849.1981. Infect Immun. 1981. PMID: 6262250 Free PMC article.
-
Strain specific antibodies revealed by immunoabsorption studies with avian infectious bronchitis virus.Vet Res Commun. 1987;11(2):109-18. doi: 10.1007/BF00344943. Vet Res Commun. 1987. PMID: 2438843 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
