[Densitometric determination of nucleic acids in gels after electrophoresis]
[Article in
Russian]
- PMID: 6205390
Item in Clipboard
[Densitometric determination of nucleic acids in gels after electrophoresis]
[Article in
Russian]
Prikl Biokhim Mikrobiol.
1984 May-Jun.
Abstract
A technique for the quantitative determining of nucleic acids after electrophoresis by double-wave densitometry in UV spectrum by scanning agar gel along both axes was developed. Optical characteristics of agar and acrylamide-agar gels are given. The range of quantities measured is 6 divided by 30 micrograms of DNA per gel; the standard deviation adjusted to 1 microgram is less than +/- 3%.
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