Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1982 Jan;92(1):53-9.
doi: 10.1083/jcb.92.1.53.

Comparative analysis of the major polypeptides from liver gap junctions and lens fiber junctions

E L Hertzberg, D J Anderson, M Friedlander, N B Gilula

Comparative analysis of the major polypeptides from liver gap junctions and lens fiber junctions

E L Hertzberg et al. J Cell Biol. 1982 Jan.

Abstract

Gap junctions from rat liver and fiber junctions from bovine lens have similar septilaminar profiles when examined by thin-section electron microscopy and differ only slightly with respect to the packing of intramembrane particles in freeze-fracture images. These similarities have often led to lens fiber junctions being referred to as gap junctions. Junctions from both sources were isolated as enriched subcellular fractions and their major polypeptide components compared biochemically and immunochemically. The major liver gap junction polypeptide has an apparent molecular weight of 27,000, while a 25,000-dalton polypeptide is the major component of lens fiber junctions. The two polypeptides are not homologous when compared by partial peptide mapping in SDS. In addition, there is not detectable antigenic similarity between the two polypeptides by immunochemical criteria using antibodies to the 25,000-dalton lens fiber junction polypeptide. Thus, in spite of the ultrastructural similarities, the gap junction and the lens fiber junction are comprised of distinctly different polypeptides, suggesting that the lens fiber junction contains a unique gene product and potentially different physiological properties.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Cell Biol. 1967 Jun;33(3):C7-C12 - PubMed
    1. J Cell Biol. 1967 May;33(2):235-42 - PubMed
    1. Nature. 1971 Jul 9;232(5306):121-2 - PubMed
    1. J Cell Biol. 1970 May;45(2):272-90 - PubMed
    1. Nature. 1972 Feb 4;235(5336):262-5 - PubMed

Publication types