Transcription of the Epstein-Barr virus genome in productively infected cells
- PMID: 6130648
- DOI: 10.1016/0042-6822(83)90286-6
Transcription of the Epstein-Barr virus genome in productively infected cells
Abstract
Transcription of the genome of Epstein-Barr virus in productively infected B95-8 cells was studied. Dot blot hybridization of cDNA prepared from cytoplasmic viral poly(A)RNA with 28 different cloned BamHI fragments of EBV DNA indicated that the patterns of viral genome transcription were similar in control and TPA-treated cells. However, three fragments, BamHI I, R, and Z, appeared to be newly induced in TPA-treated cells, as no significant level of transcription of these fragments was observed in control cells. In contrast, there was no detectable transcription from fragments BamHI P, a, and b even in cells treated with TPA. The size of virus-specific RNA was determined by agarose gel electrophoresis followed by Southern blot hybridization using radioactive BamHI cloned EBV DNA fragments as probes. Sixty-eight cytoplasmic poly(A)RNA species, ranging in size from 0.1 to 2.8 megadaltons, were detected by this procedure.
Similar articles
-
Phosphonoacetic acid-resistant RNA of Epstein-Barr virus in productively infected cells.Virology. 1983 Jan 15;124(1):196-200. doi: 10.1016/0042-6822(83)90306-9. Virology. 1983. PMID: 6130649
-
Transcription and DNA sequence of the BamHI L fragment of B95-8 Epstein-Barr virus.EMBO J. 1984 May;3(5):1083-90. doi: 10.1002/j.1460-2075.1984.tb01933.x. EMBO J. 1984. PMID: 6203743 Free PMC article.
-
Epstein-Barr virus RNA. VIII. Viral RNA in permissively infected B95-8 cells.J Virol. 1982 Jul;43(1):262-72. doi: 10.1128/JVI.43.1.262-272.1982. J Virol. 1982. PMID: 6180174 Free PMC article.
-
Organization of the Epstein-Barr virus DNA molecule. III. Location of the P3HR-1 deletion junction and characterization of the NotI repeat units that form part of the template for an abundant 12-O-tetradecanoylphorbol-13-acetate-induced mRNA transcript.J Virol. 1983 Oct;48(1):135-48. doi: 10.1128/JVI.48.1.135-148.1983. J Virol. 1983. PMID: 6310141 Free PMC article.
-
Mapping of putative transforming sequences of EBV DNA.IARC Sci Publ (1971). 1978;(24 Pt 1):527-52. IARC Sci Publ (1971). 1978. PMID: 221358 Review.
Cited by
-
The BHLF1 Locus of Epstein-Barr Virus Contributes to Viral Latency and B-Cell Immortalization.J Virol. 2020 Aug 17;94(17):e01215-20. doi: 10.1128/JVI.01215-20. Print 2020 Aug 17. J Virol. 2020. PMID: 32581094 Free PMC article.
-
Latent and viral replicative transcription in vivo from the BamHI K fragment of Epstein-Barr virus DNA.J Virol. 1985 May;54(2):501-8. doi: 10.1128/JVI.54.2.501-508.1985. J Virol. 1985. PMID: 2985812 Free PMC article.
-
Accelerated onset of viral transcription in adenovirus-infected HeLa cells treated with the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate.Mol Cell Biol. 1984 Mar;4(3):563-6. doi: 10.1128/mcb.4.3.563-566.1984. Mol Cell Biol. 1984. PMID: 6717433 Free PMC article.
-
Both the rightward and the leftward open reading frames within the BamHI M DNA fragment of Epstein-Barr virus act as trans-activators of gene expression.J Virol. 1987 Oct;61(10):3310-3. doi: 10.1128/JVI.61.10.3310-3313.1987. J Virol. 1987. PMID: 3041051 Free PMC article.
-
The expression of novel antigens from the Epstein-Barr virus large internal repeat.EMBO J. 1988 Apr;7(4):1191-6. doi: 10.1002/j.1460-2075.1988.tb02930.x. EMBO J. 1988. PMID: 2841116 Free PMC article.