Comparative Study
doi: 10.1089/dna.1.1984.3.469.
Oligonucleotide-directed mutagenesis using plasmid DNA templates and two primers
- PMID: 6096100
- DOI: 10.1089/dna.1.1984.3.469
Item in Clipboard
Comparative Study
Oligonucleotide-directed mutagenesis using plasmid DNA templates and two primers
DNA.
1984 Dec.
Abstract
Oligonucleotide-directed mutagenesis using plasmid vectors has been simplified by introducing two changes to the previous method. First, template preparation has been simplified by using the covalently closed circular plasmid DNA directly for mutagenesis, eliminating the need for a wholly or partially single-stranded circular DNA template. Second, two primers are used, eliminating the need for producing the covalently closed molecule during in vitro replication. The advantages of the approach are discussed.
Similar articles
-
Site-directed mutagenesis by complementary-strand synthesis using a closing oligonucleotide and double-stranded DNA templates.Anal Biochem. 1990 Feb 15;185(1):194-200. doi: 10.1016/0003-2697(90)90279-i. Anal Biochem. 1990. PMID: 2111641
-
Site-directed mutagenesis of virtually any plasmid by eliminating a unique site.Anal Biochem. 1992 Jan;200(1):81-8. doi: 10.1016/0003-2697(92)90280-k. Anal Biochem. 1992. PMID: 1595905
-
Primer-directed mutagenesis of linearized plasmids.Anal Biochem. 1984 Dec;143(2):341-9. doi: 10.1016/0003-2697(84)90673-0. Anal Biochem. 1984. PMID: 6397999
-
Processing of plasmid DNA during bacterial conjugation.Microbiol Rev. 1984 Mar;48(1):24-41. doi: 10.1128/mr.48.1.24-41.1984. Microbiol Rev. 1984. PMID: 6201705 Free PMC article. Review. No abstract available.
-
[Directed mutagenesis].Genetika. 1982 Mar;18(3):349-59. Genetika. 1982. PMID: 6281129 Review. Russian.
Cited by
-
Use of oligonucleotides and nick translation for site-directed mutagenesis in plasmids.Nucleic Acids Res. 1992 Feb 25;20(4):922. doi: 10.1093/nar/20.4.922. Nucleic Acids Res. 1992. PMID: 1542589 Free PMC article. No abstract available.
-
Differentiation of species and strains among filamentous fungi by DNA fingerprinting.Curr Genet. 1991 Mar;19(3):239-42. doi: 10.1007/BF00336493. Curr Genet. 1991. PMID: 1907892
-
Direct demonstration of critical amino acid residues required for cytotoxic T-lymphocyte allorecognition of H-2 class I antigens.Proc Natl Acad Sci U S A. 1988 May;85(9):3085-9. doi: 10.1073/pnas.85.9.3085. Proc Natl Acad Sci U S A. 1988. PMID: 2452442 Free PMC article.
-
A variant octamer motif in a Xenopus H2B histone gene promoter is not required for transcription in frog oocytes.Mol Cell Biol. 1991 Feb;11(2):641-54. doi: 10.1128/mcb.11.2.641-654.1991. Mol Cell Biol. 1991. PMID: 1990276 Free PMC article.
-
Transformation of yeast with synthetic oligonucleotides.Proc Natl Acad Sci U S A. 1988 Jan;85(2):524-8. doi: 10.1073/pnas.85.2.524. Proc Natl Acad Sci U S A. 1988. PMID: 2829192 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Other Literature Sources