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. 1984 Oct;160(1):175-83.
doi: 10.1128/jb.160.1.175-183.1984.

Construction of a tyrP-lac operon fusion strain and its use in the isolation and analysis of mutants derepressed for tyrP expression

P A Kasian, J Pittard

Construction of a tyrP-lac operon fusion strain and its use in the isolation and analysis of mutants derepressed for tyrP expression

P A Kasian et al. J Bacteriol. 1984 Oct.

Abstract

The gene tyrP, which codes for a component of the tyrosine-specific transport system, has been localized on the Escherichia coli K-12 chromosome at min 42. A tyrP-lac operon fusion was constructed and used to isolate mutants that have altered expression from the tyrP promoter. All putative tyrP operator mutations were transferred onto a plasmid vector by recombination in vivo. Restriction enzyme analysis of the resultant plasmids suggests that some of these mutants arose from either an insertion or a deletion of DNA occurring within the region of DNA that contains the tyrP promoter.

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