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. 1984 Aug;38(1):309-16.
doi: 10.1016/0092-8674(84)90552-x.

Trypanosome mRNAs share a common 5' spliced leader sequence

M Parsons, R G Nelson, K P Watkins, N Agabian

Trypanosome mRNAs share a common 5' spliced leader sequence

M Parsons et al. Cell. 1984 Aug.

Abstract

A 5'-terminal leader sequence of 35 nucleotides was found to be present on multiple trypanosome RNAs. Based on its representation in cDNA libraries, we estimate that many, if not all, trypanosome mRNAs contain this leader. This same leader was originally identified on mRNAs encoding the molecules responsible for antigenic variation, variant surface glycoproteins. Studies of selected cDNAs containing this leader sequence revealed that leader-containing transcripts can be stage-specific, stage-regulated, or constitutive. They can be abundant or rare, and transcribed from single or multigene families. No linkage between the genomic leader sequences and the structural gene exons was observed. Possible mechanisms by which the leader sequences are added to trypanosome mRNAs are discussed.

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References

    1. Agabian N., Thomashow L., Milhausen M., Stuart K. Structural analysis of variant and invariant genes in trypanosomes. Am. J. Trop. Med. Hyg. 1980;29(5):1043–1049. Suppl. - PubMed
    1. Bernards A., Van der Ploeg L.H.T., Frasch A.C.C., Borst P., Boothroyd J.C., Coleman S., Cross G.A.M. Activation of trypanosome surface glycoprotein genes involves a duplication-transposition leading to an altered 3′ end. Cell. 1981;27:497–505. - PubMed
    1. Bernards A., Michels P., Lincke C., Borst P. Growth of chromosome ends in multiplying trypanosomes. Nature. 1983;303:592–597. - PubMed
    1. Boothroyd J., Cross G. Transcripts coding for variant surface glycoproteins of Trypanosoma brucei have a short, identical exon at their 5′ end. Gene. 1982;20:281–289. - PubMed
    1. Buck G., Longacre S., Raibaud A., Hibner U., Giroud C., Baltz T., Baltz D., Eisen H. Stability of expression-linked surface antigen gene in Trypanosoma equiperdum. Nature. 1984;307:563–566. - PubMed

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