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. 1978 Nov;28(2):466-74.
doi: 10.1128/JVI.28.2.466-474.1978.

Mutants of Sindbis virus. III. Host polypeptides present in purified HR and ts103 virus particles

E G Strauss

Mutants of Sindbis virus. III. Host polypeptides present in purified HR and ts103 virus particles

E G Strauss. J Virol. 1978 Nov.

Abstract

The amounts of host-encoded protein present in purified Sindbis virions of both the HR strain and of a mutant (ts103) which makes multicored particles were examined. Cells were labeled with [35S]methionine before infection and with [3H]methionine postinfection. Virions were purified by velocity sedimentation and isopycnic banding, and their polypeptides were examined by polyacrylamide gels in a sodium dodecyl sulfate-containing discontinuous buffer system. Host prelabeled material was found principally in a small number of discrete polypeptides in HR virions, which contained as little as 0.2% host-encoded protein. Virus-sized particles of mutant ts103 contained significantly more host material, and multiploid particles from ts103 infection contained up to 12% host prelabeled protein.

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