The proteins of bacteriophage M13
- PMID: 5257006
- PMCID: PMC223669
- DOI: 10.1073/pnas.62.3.800
The proteins of bacteriophage M13
Abstract
Particles of the small filamentous coliphage M13 contain not only the major coat protein, which is the product of phage gene 8, but also a minor coat protein, the A protein, which is the product of gene 3. The A protein has a molecular weight of approximately 70,000 daltons, is present in one copy per virion, and is responsible for phage attachment to host cells. Also associated with purified M13 particles is a minor quantity of very small proteinaceous material, but its origin as a phage-coded product has not been demonstrated. At least five phage-specific proteins, including the two coat proteins, are present in appreciable quantities in M13-infected cells. The principal phage protein synthesized is the product of gene 5, which is responsible for phage single-stranded DNA synthesis. This protein has a molecular weight of about 8,000 daltons. Its precise function in DNA synthesis is not yet known. Phage proteins are synthesized at nearly normal rates in cells in which replication of phage double-stranded DNA is blocked by gene 2 mutations. This result suggests that the initial double-stranded DNA molecule serves as the principal template, perhaps the only template, for phage messenger RNA synthesis.
Similar articles
-
A coat protein of the bacteriophage M13 virion participates in membrane-oriented synthesis of DNA.Proc Natl Acad Sci U S A. 1973 Jan;70(1):205-9. doi: 10.1073/pnas.70.1.205. Proc Natl Acad Sci U S A. 1973. PMID: 4567335 Free PMC article.
-
Replication of bacteriophage M13: specificity of the Escherichia coli dnaB function for replication of double-stranded M13 DNA.Proc Natl Acad Sci U S A. 1972 Sep;69(9):2570-3. doi: 10.1073/pnas.69.9.2570. Proc Natl Acad Sci U S A. 1972. PMID: 4560691 Free PMC article.
-
Gene V protein-mediated translational regulation of the synthesis of gene II protein of the filamentous bacteriophage M13: a dispensable function of the filamentous-phage genome.J Bacteriol. 1992 Jan;174(2):595-600. doi: 10.1128/jb.174.2.595-600.1992. J Bacteriol. 1992. PMID: 1729248 Free PMC article.
-
Uniqueness of RNA Coliphage Qβ Display System in Directed Evolutionary Biotechnology.Viruses. 2021 Mar 27;13(4):568. doi: 10.3390/v13040568. Viruses. 2021. PMID: 33801772 Free PMC article. Review.
-
Structural constraints on the display of foreign peptides on filamentous bacteriophages.Gene. 1993 Jun 15;128(1):5-11. doi: 10.1016/0378-1119(93)90146-t. Gene. 1993. PMID: 8508959 Review.
Cited by
-
Nanomechanics of graphene oxide-bacteriophage based self-assembled porous composites.Sci Rep. 2020 Sep 24;10(1):15618. doi: 10.1038/s41598-020-72372-1. Sci Rep. 2020. PMID: 32973218 Free PMC article.
-
Mechanisms of membrane assembly: effects of energy poisons on the conversion of soluble M13 coliphage procoat to membrane-bound coat protein.Proc Natl Acad Sci U S A. 1980 Feb;77(2):827-31. doi: 10.1073/pnas.77.2.827. Proc Natl Acad Sci U S A. 1980. PMID: 6928682 Free PMC article.
-
Ricin detection using phage displayed single domain antibodies.Sensors (Basel). 2009;9(1):542-55. doi: 10.3390/s90100542. Epub 2009 Jan 19. Sensors (Basel). 2009. PMID: 22389616 Free PMC article.
-
Synthesis of the major bacteriophage fl coat protein.J Virol. 1971 Jun;7(6):776-82. doi: 10.1128/JVI.7.6.776-782.1971. J Virol. 1971. PMID: 4935446 Free PMC article.
-
The amino acid sequence of the B-protein of bacteriophage ZJ-2.Biochem J. 1972 Mar;127(1):167-78. doi: 10.1042/bj1270167. Biochem J. 1972. PMID: 5073740 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
