Composition and spectral properties of a cytochrome-enriched fraction obtained from chloroplasts by digitonin treatment
- PMID: 508303
- PMCID: PMC1161344
- DOI: 10.1042/bj1820613
Composition and spectral properties of a cytochrome-enriched fraction obtained from chloroplasts by digitonin treatment
Abstract
A cytochrome-enriched fraction obtained from chloroplasts after treatment with the detergent digitonin contained cytochromes f, b-559LP and b-563 in the approximate proportions 1:1:2, close to those observed in unfractionated chloroplasts. The spectrum of cytochrome b-563 at temperature of liquid N2 showed a single asymmetrical alpha-band with a maximum at 561 nm.
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