Structure and function of bacterial ribosomes. XI. Dependence of 50S ribosomal assembly on simultaneous assembly of 30S subunits
- PMID: 4922290
- PMCID: PMC283371
- DOI: 10.1073/pnas.67.3.1440
Structure and function of bacterial ribosomes. XI. Dependence of 50S ribosomal assembly on simultaneous assembly of 30S subunits
Abstract
Some spectinomycin-resistant mutants of Escherichia coli are cold-sensitive. They are unable to assemble both 30S and 50S ribosomal subunits at low temperatures. They accumulate two kinds of incomplete particles, related to 30S and 50S subunits respectively. A single mutation, causing an alteration in a 30S ribosomal component, is responsible for these phenotypes. These results show that assembly of 50S subunits in vivo is dependent on simultaneous assembly of 30S subunits. On the other hand, the assembly of 30S subunits appears to be independent of 50S assembly.
Similar articles
-
Functional interdependence of 50S and 30S ribosomal subunits.Mol Gen Genet. 1974;135(1):11-8. doi: 10.1007/BF00433896. Mol Gen Genet. 1974. PMID: 4280506 No abstract available.
-
Binding of erythromycin to the 50S ribosomal subunit is affected by alterations in the 30S ribosomal subunit.Mol Gen Genet. 1976 Feb 2;143(3):301-6. doi: 10.1007/BF00269407. Mol Gen Genet. 1976. PMID: 765762
-
Ribosomal proteins involved in the suppression of streptomycin dependence in Escherichia coli.J Bacteriol. 1972 Feb;109(2):780-5. doi: 10.1128/jb.109.2.780-783.1972. J Bacteriol. 1972. PMID: 4258063 Free PMC article.
-
[Assembly of the 30S ribosome subunit in E. coli].Tanpakushitsu Kakusan Koso. 1971 Jul;16(7):483-91. Tanpakushitsu Kakusan Koso. 1971. PMID: 4934332 Review. Japanese. No abstract available.
-
[Mutants in ribosome assembly].Tanpakushitsu Kakusan Koso. 1972;17:Suppl:134-46. Tanpakushitsu Kakusan Koso. 1972. PMID: 4559521 Review. Japanese. No abstract available.
Cited by
-
A genetic and biochemical study of streptomycin- and spectinomycin-resistance in Salmonella typhimurium.Mol Gen Genet. 1971;110(3):197-210. doi: 10.1007/BF00337833. Mol Gen Genet. 1971. PMID: 4253147 No abstract available.
-
Chloramphenicol resistance mutation in Escherichia coli which maps in the major ribosomal protein gene cluster.J Bacteriol. 1979 Mar;137(3):1315-23. doi: 10.1128/jb.137.3.1315-1323.1979. J Bacteriol. 1979. PMID: 374348 Free PMC article.
-
Cold-sensitive mutants in Aspergillus nidulans. II. Mutations affecting ribosome production.Mol Gen Genet. 1974;134(2):115-32. doi: 10.1007/BF00268414. Mol Gen Genet. 1974. PMID: 4617158 No abstract available.
-
Interaction between the ribosomal subunits: 16S rRNA suppressors of the lethal DeltaA1916 mutation in the 23S rRNA of Escherichia coli.Mol Genet Genomics. 2007 Sep;278(3):307-15. doi: 10.1007/s00438-007-0252-y. Epub 2007 Jun 13. Mol Genet Genomics. 2007. PMID: 17564727
-
Assembly of 60S ribosomal subunits is perturbed in temperature-sensitive yeast mutants defective in ribosomal protein L16.Mol Cell Biol. 1991 Nov;11(11):5681-92. doi: 10.1128/mcb.11.11.5681-5692.1991. Mol Cell Biol. 1991. PMID: 1922070 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources