Relationship between Fc receptors, antigen-binding sites on T and B cells, and H-2 complex-associated determinants
- PMID: 46915
- PMCID: PMC2189703
- DOI: 10.1084/jem.141.3.547
Relationship between Fc receptors, antigen-binding sites on T and B cells, and H-2 complex-associated determinants
Abstract
The relationship between H-2 complex-associated determinants, Fc receptors, and specific antigen-recognition sites on T and B cells was examined by binding and functional assays. The Fc receptor was detected by radiolabeled immune complexes or aggregated human IgG. Both these reagents selectively bound to B cells, not to T cells. When spleen cells, from mice primed to several antigens, were exposed to highly substituted radioactive aggregates, their capacity to transfer both a direct and indirect plaque-forming cell response to these antigens was abrogated. Addition of B cells, but not of T cells, restored responsiveness. Complexed Ig binding to Fc receptors was prevented by pretreatment of mixed lymphoid cell populations with antisera directed against membrane components on the same cell (e.g., H-2) and on other cells (e.g., theta). The lack of specificity of inhibition was thought to be due to the formation on cell surfaces of antigen-antibody complexes which would then attach to the Fc receptor during the incubation precedure. Specific blockade of the Fc receptor during the incubation procedure. Specific blockade of the Fc receptor however occurred when B cells were pretreated with the Fab fragments of anti-H-2 antibody. This was demonstrated autoradiographically and by inhibition of aggregate-induced suicide. The blocking activity of ante-H-2 Fab was removed by absorption with spleen cells from thymectomized irradiated mice but not with thymus cells of appropriate specificity. This suggested that the antibodies involved had specificity for determinants on the B-cell membrane distinct from those coded by the K or D end of the H-2 complex, and either absent from, or poorly represented on, thymus cells. Specific antigen-induced suicide of B cells was achieved simply by incubating the cells with radioactive antigen in the cold. T-cell suicide on the other hand required that the 125I-labeled antigen be presented to the T cells at 37 degrees-C on the surface of spleen cells from antigen-primed mice. Pretreatment of T cells with the Fab fragment of anti-H-2 antibody protected them from the suicide effect. By contrast no such protection of B cells could be achieved by this procedure. In other words H-2 (? Ir)-associated determinants may not only be in close proximity to the antigen-binding site on T cells but, in addition, may be involved in the effective operation of the receptor.
Similar articles
-
Evidence for identity or close association of the Fc receptor of B lymphocytes and alloantigens determined by the Ir region of the H-2 complex.J Exp Med. 1974 Sep 1;140(3):779-96. doi: 10.1084/jem.140.3.779. J Exp Med. 1974. PMID: 4137653 Free PMC article.
-
Interactions of Fc receptors with antibodies against Ia antigens and other cell surface components.J Exp Med. 1975 May 1;141(5):1201-9. doi: 10.1084/jem.141.5.1201. J Exp Med. 1975. PMID: 1079233 Free PMC article.
-
Mouse thymus-independent and thymus-derived lymphoid cells. I. Immunofluorescent and functional studies.J Exp Med. 1972 Nov 1;136(5):984-1007. doi: 10.1084/jem.136.5.984. J Exp Med. 1972. PMID: 4117194 Free PMC article.
-
Lymphocyte surface receptors and leukemia virus-induced immunosuppression.Am J Clin Pathol. 1975 May;63(5):735-47. doi: 10.1093/ajcp/63.5.735. Am J Clin Pathol. 1975. PMID: 1093386 Review.
-
Lymphocyte receptors for immunoglobulin.Adv Immunol. 1976;24:167-214. doi: 10.1016/s0065-2776(08)60330-2. Adv Immunol. 1976. PMID: 798474 Review. No abstract available.
Cited by
-
Recognition of antigens by T lymphocytes.Mol Cell Biochem. 1978 Dec 22;22(2-3):93-112. doi: 10.1007/BF00496237. Mol Cell Biochem. 1978. PMID: 85255 Review.
-
Detection of non-H2 antigen(s) which, like Ia antigens, are associated with the Fc receptor of B lymphocytes.J Exp Med. 1975 Sep 1;142(3):796-801. doi: 10.1084/jem.142.3.796. J Exp Med. 1975. PMID: 1100765 Free PMC article.
-
Macrophage-T cell interaction mediated by immunogenic and non-immunogenic forms of a monofunctional antigen.Mol Cell Biochem. 1979 Jun 15;25(3):131-42. doi: 10.1007/BF00235363. Mol Cell Biochem. 1979. PMID: 314564
-
Different rosette assays for detecting Fc receptor-bearing lymphocytes measure different subpopulations.Immunology. 1978 Aug;35(2):407-14. Immunology. 1978. PMID: 312254 Free PMC article.
-
Immunocompetent cells in resistance to bacterial infections.Bacteriol Rev. 1976 Jun;40(2):284-313. doi: 10.1128/br.40.2.284-313.1976. Bacteriol Rev. 1976. PMID: 786253 Free PMC article. Review. No abstract available.