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. 1973 May;70(5):1293-7.
doi: 10.1073/pnas.70.5.1293.

Recircularization and autonomous replication of a sheared R-factor DNA segment in Escherichia coli transformants

S N Cohen, A C Chang

Recircularization and autonomous replication of a sheared R-factor DNA segment in Escherichia coli transformants

S N Cohen et al. Proc Natl Acad Sci U S A. 1973 May.

Abstract

Controlled shearing of R-factor DNA leads to formation of fragments carrying an antibiotic resistance gene present on, but not expressed by, the intact R-factor. Transformation of CaCl(2)-treated E. coli by such fragments yields an autonomously replicating tetracycline-resistance plasmid (Tc6-5) that contains only a small fraction of the genome of the parent R-factor, and lacks both its fertility functions and its other drug-resistance determinants. Although the Tc6-5 plasmid is not self-transmissible, it can interact and/or recombine with conjugally-proficient plasmids that promote its transfer to other bacteria.

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References

    1. Nature. 1965 Nov 27;208(5013):843-9 - PubMed
    1. J Mol Biol. 1967 May 28;26(1):19-38 - PubMed
    1. Nature. 1967 Nov 18;216(5116):647-52 - PubMed
    1. Proc Natl Acad Sci U S A. 1968 May;60(1):243-50 - PubMed
    1. Proc Natl Acad Sci U S A. 1968 Jul;60(3):992-9 - PubMed

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