Defective deoxyribonucleic acid replication of T4rII bacteriophage in lambda-lysogenic host cells
- PMID: 4561406
- PMCID: PMC356463
- DOI: 10.1128/JVI.10.2.310-313.1972
Defective deoxyribonucleic acid replication of T4rII bacteriophage in lambda-lysogenic host cells
Abstract
Sedimentation of the replicative deoxyribonucleic acid through alkaline sucrose gradients showed that rII single chains reached the half-mature size at a time when wild-type molecules formed long chains (dimers and trimers of genome size). Long rII single chains could be observed on substitution of tris(hydroxymethyl)aminomethane buffer for Na(+)K(+) phosphate in the growth medium.
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