A particle associated with the polyadenylate segment in mammalian messenger RNA
- PMID: 4508317
- PMCID: PMC389746
- DOI: 10.1073/pnas.69.11.3247
A particle associated with the polyadenylate segment in mammalian messenger RNA
Abstract
A structure consisting of poly(A) complexed with other components is released from polysomes by ribonuclease treatment. The poly(A) complex has a sedimentation value of 12-15, while the corresponding sedimentation value for free poly(A) is 4. The complex does not appear to represent an artifact formed by interaction of free poly(A) with either cytoplasmic or polysomal proteins. The polynucleotide released from the complex by treatment with sodium dodecyl sulfate shows the same electrophoretic mobility as that of poly(A) isolated from deproteinized polysomal RNA. The poly(A) in the complex is partially protected from digestion by T(2) ribonuclease. At least part of the poly(A) is available for base pairing with poly(U). The components associated with the poly(A) cause it to bind to Millipore filters at low ionic strength. These components are removed from the complex by Pronase digestion. The findings indicate that the poly(A) segment in messenger RNA serves as a binding site for a particle. This particle appears to consist of proteins.
Similar articles
-
Association of the polyadenylate segment of messenger RNA with other polynucleotide sequences in mouse sarcoma 180 polyribosomes.Biochemistry. 1975 Jul 29;14(15):3445-51. doi: 10.1021/bi00686a024. Biochemistry. 1975. PMID: 1148210
-
Cleavage of the polyadenylate-rich region of polyadenylate-rich RNA.Biochem Biophys Res Commun. 1972 Oct 6;49(1):230-8. doi: 10.1016/0006-291x(72)90034-4. Biochem Biophys Res Commun. 1972. PMID: 4672928 No abstract available.
-
Isolation and hybridization kinetics of messenger RNA from Dictyostelium discoideum.Nat New Biol. 1972 Oct 25;239(95):225-8. doi: 10.1038/newbio239225a0. Nat New Biol. 1972. PMID: 4507801 No abstract available.
-
A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells.Proc Natl Acad Sci U S A. 1971 Jun;68(6):1331-5. doi: 10.1073/pnas.68.6.1331. Proc Natl Acad Sci U S A. 1971. PMID: 5288382 Free PMC article.
-
The isolation of RNA from mammalian cells.Methods Cell Biol. 1973;7:1-22. doi: 10.1016/s0091-679x(08)61769-0. Methods Cell Biol. 1973. PMID: 4592521 Review. No abstract available.
Cited by
-
Complex formation between poly-r (U) and various chromosomal loci in Rhynchosciara.Chromosoma. 1973;43(4):375-90. doi: 10.1007/BF00406744. Chromosoma. 1973. PMID: 4776471 No abstract available.
-
Effect of homopolyribonucleotides on messenger ribonucleoprotein particles.Nucleic Acids Res. 1977 Apr;4(4):917-28. doi: 10.1093/nar/4.4.917. Nucleic Acids Res. 1977. PMID: 866198 Free PMC article.
-
Polyamines, ribonucleases, and the stability of RNA.Mol Cell Biochem. 1977 Sep 9;17(2):89-99. doi: 10.1007/BF01743432. Mol Cell Biochem. 1977. PMID: 333273 Review. No abstract available.
-
Poly(A) shortening and degradation of the 3' A+U-rich sequences of human c-myc mRNA in a cell-free system.Mol Cell Biol. 1988 Apr;8(4):1697-708. doi: 10.1128/mcb.8.4.1697-1708.1988. Mol Cell Biol. 1988. PMID: 3380094 Free PMC article.
-
Metabolism of Poly(A) in Plant Cells: Discrete Classes Associated with Free and Membrane-bound Polysomes.Plant Physiol. 1976 Sep;58(3):405-10. doi: 10.1104/pp.58.3.405. Plant Physiol. 1976. PMID: 16659688 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
