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. 1979 Apr;6(4):1479-93.
doi: 10.1093/nar/6.4.1479.

Conformation of DNA in chromatin protein-DNA complexes studied by infrared spectroscopy

J Liquier, M C Gadenne, E Taillandier, N Defer, F Favatier, J Kruh
Free PMC article

Conformation of DNA in chromatin protein-DNA complexes studied by infrared spectroscopy

J Liquier et al. Nucleic Acids Res. 1979 Apr.
Free PMC article

Abstract

The following observations concerning the DNA secondary structures in various nucleohistone complexes were made by infrared spectroscopy: 1/ in chromatin, chromatin extracted by 0.6 M NaCl, nucleosomes, and histone-DNA reconstituted complexes, the DNA remains in a B type conformation at low relative hygrometry; 2/ in chromatin extracted by tRNA and in non histone protein-DNA reconstituted complexes, the DNA can adopt an A type conformation. Infrared linear dichroism data show that in NHP-DNA complexes the low relative hygrometry conformation of DNA may be modified and that the infrared parameter -1090 is close to that measured for RNA's or DNA-RNA hybrids. It is concluded that the histones block the DNA in a B form and that some of the NHP could be involved in the control of the secondary structure of DNA in chromatin.

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