Sequence relationships among the variable regions of immunoglobulin heavy chains from various mammalian species
- PMID: 4506072
- PMCID: PMC426866
- DOI: 10.1073/pnas.69.8.2052
Sequence relationships among the variable regions of immunoglobulin heavy chains from various mammalian species
Abstract
Immunoglobulin heavy chains of myeloma proteins from dogs and cata have been subjected to automated sequence analysis. When the results were compared with human heavy-chain sequences, all the dog and cat proteins could be unequivocally assigned to the V(H)III subgroup. This pattern contrasts with that in human proteins in which only 25% of all heavy chains sequenced belong to this subgroup. The 30 residues at the NH(2) termini of dog, cat, and human heavy chains had sequence identities near or exceeding 90%, in contrast to established interspecies sequence homologies of constant regions of about 60%. Some genes of the immunoglobulin heavy-chain variable region thus appear to have been conserved through a considerable period of evolutionary time. The analyses also showed that the presence of certain amino acids at certain positions in these heavy chains could be correlated with the species of origin. The occurrence of such "phylogenetically associated" residues is most consistent with the presence of a restricted number of genes in the heavy-chain variable region pool.
Similar articles
-
Variable region sequences of five human immunoglobulin heavy chains of the VH3 subgroup: definitive identification of four heavy chain hypervariable regions.Proc Natl Acad Sci U S A. 1974 Mar;71(3):845-8. doi: 10.1073/pnas.71.3.845. Proc Natl Acad Sci U S A. 1974. PMID: 4522793 Free PMC article.
-
Heavy-chain variable regions in normal and pathological immunolobulins.Proc Natl Acad Sci U S A. 1971 Jun;68(6):1143-6. doi: 10.1073/pnas.68.6.1143. Proc Natl Acad Sci U S A. 1971. PMID: 5289298 Free PMC article.
-
Distribution and association of heavy and light chain variable region subgroups among human IgA immunoglobulins.J Immunol. 1975 Feb;114(2 Pt 1):678-81. J Immunol. 1975. PMID: 804511
-
The immunoglobulins of the cat.Cornell Vet. 1981 Oct;71(4):397-407. Cornell Vet. 1981. PMID: 6797789 Review. No abstract available.
-
The J chain of polymeric immunoglobulins.Contemp Top Mol Immunol. 1974;3:111-41. doi: 10.1007/978-1-4684-2838-4_5. Contemp Top Mol Immunol. 1974. PMID: 4215614 Review. No abstract available.
Cited by
-
Phylogenetically associated residues within the VH3 subgroup of several mammalian species. Evidence for a "pauci-gene" basis for antibody diversity.J Exp Med. 1973 Aug 1;138(2):410-27. doi: 10.1084/jem.138.2.410. J Exp Med. 1973. PMID: 4198201 Free PMC article.
-
Amino-acid sequence of the variable region of the heavy (alpha) chain of a mouse myeloma protein with anti-hapten activity.Proc Natl Acad Sci U S A. 1974 Apr;71(4):1123-7. doi: 10.1073/pnas.71.4.1123. Proc Natl Acad Sci U S A. 1974. PMID: 4524622 Free PMC article.
-
Evolution of immunoglobulin heavy chain variable region genes: a VH family can last for 150-200 million years or longer.Immunogenetics. 1995;41(1):18-28. doi: 10.1007/BF00188428. Immunogenetics. 1995. PMID: 7806270
-
Completion of the analysis of the primary structure of the variable domain of a homogeneous rabbit antibody to type III pneumococcal polysaccharide.Biochem J. 1974 Dec;143(3):723-32. doi: 10.1042/bj1430723. Biochem J. 1974. PMID: 4142749 Free PMC article.
-
Variable region sequences of five human immunoglobulin heavy chains of the VH3 subgroup: definitive identification of four heavy chain hypervariable regions.Proc Natl Acad Sci U S A. 1974 Mar;71(3):845-8. doi: 10.1073/pnas.71.3.845. Proc Natl Acad Sci U S A. 1974. PMID: 4522793 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
