Characterization of a unique muscle cell line
- PMID: 4363958
- PMCID: PMC2109296
- DOI: 10.1083/jcb.61.2.398
Characterization of a unique muscle cell line
Abstract
A clonal cell line derived from a mouse neoplasm is described which shares many properties with smooth muscle. The cells have electrically excitable membranes capable of generating overshooting action potentials, and they contract both spontaneously and with electrical stimulation. They respond to the iontophoretic application of acetylcholine with a depolarizing response, and to norepinephrine with a hyperpolarizing response. Electron microscopy reveals that the cells have a morphology similar in many, but not all, respects to that of smooth muscle cells in vivo. The cells secrete soluble collagen-like molecules in addition to several proteins of undefined function. Finally, there is an increase in the specific activities of creatine phosphokinase and myokinase associated with increased cell density and the cessation of cell division.
Similar articles
-
Development of a clonal myogenic cell line with unusual biochemical properties.J Cell Physiol. 1976 Jul;88(3):255-75. doi: 10.1002/jcp.1040880302. J Cell Physiol. 1976. PMID: 178672
-
Chemosensitivity of single smooth muscle cells to acetylcholine, noradrenaline, and histamine in vitro.J Cell Physiol. 1980 Feb;102(2):99-112. doi: 10.1002/jcp.1041020202. J Cell Physiol. 1980. PMID: 6246131
-
Characterization of two putative smooth muscle cell lines from rat thoracic aorta.Exp Cell Res. 1976 Mar 15;98(2):349-66. doi: 10.1016/0014-4827(76)90446-8. Exp Cell Res. 1976. PMID: 943301 No abstract available.
-
Modulation of smooth muscle by nervous activity: a review and a hypothesis.Fed Proc. 1977 Sep;36(10):2456-61. Fed Proc. 1977. PMID: 196945 Review. No abstract available.
-
Proliferative disorders of myofibroblasts.Ultrastruct Pathol. 1993 May-Aug;17(3-4):211-20. doi: 10.3109/01913129309027767. Ultrastruct Pathol. 1993. PMID: 8266586 Review. No abstract available.
Cited by
-
Some observations on the fine structure of the myotendinous junction in myotomal muscle of the tadpole tail.Cell Tissue Res. 1976 Feb 12;166(2):241-54. doi: 10.1007/BF00227045. Cell Tissue Res. 1976. PMID: 1248047
-
Autonomous expression of c-myc in BC3H1 cells partially inhibits but does not prevent myogenic differentiation.Mol Cell Biol. 1987 May;7(5):1973-7. doi: 10.1128/mcb.7.5.1973-1977.1987. Mol Cell Biol. 1987. PMID: 3299056 Free PMC article.
-
Molecular cloning and characterization of the ryanodine receptor/junctional channel complex cDNA from skeletal muscle sarcoplasmic reticulum.Proc Natl Acad Sci U S A. 1989 Nov;86(22):8683-7. doi: 10.1073/pnas.86.22.8683. Proc Natl Acad Sci U S A. 1989. PMID: 2813419 Free PMC article.
-
Muscle cell differentiation is associated with increased insulin receptor biosynthesis and messenger RNA levels.J Clin Invest. 1989 Jan;83(1):192-8. doi: 10.1172/JCI113858. J Clin Invest. 1989. PMID: 2910907 Free PMC article.
-
"In situ" translation: use of the cytoskeletal framework to direct cell-free protein synthesis.In Vitro Cell Dev Biol. 1991 Jan;27(1):75-85. doi: 10.1007/BF02630897. In Vitro Cell Dev Biol. 1991. PMID: 2013556
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
