Fusion of erythrocytes by Sendai virus studied by immuno-freeze-etching
- PMID: 4351454
- PMCID: PMC355210
- DOI: 10.1128/JVI.11.6.1004-1012.1973
Fusion of erythrocytes by Sendai virus studied by immuno-freeze-etching
Abstract
Extensive fusion of human erythrocytes agglutinated by Sendai virus was observed after 30 s of incubation at 37 C. Electron microscopy of thin sections failed to reveal the presence of virions, viral fragments, or discrete viral antigens reactive with ferritin-labeled antibody at the sites of fusion. Immuno-freezeetching of membrane surfaces demonstrated the dispersal of viral envelope antigens from what appeared to be original sites of viral attachment. Virus-induced clustering of membrane glycoproteins was interpreted as resulting from interaction of viral antigens with membrane receptor proteins and forming the structural basis for fusion of membranes with one another.
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