doi: 10.1083/jcb.63.3.1018.
Intramembrane particle aggregation in erythrocyte ghosts. I. The effects of protein removal
- PMID: 4215819
- PMCID: PMC2109372
- DOI: 10.1083/jcb.63.3.1018
Item in Clipboard
Intramembrane particle aggregation in erythrocyte ghosts. I. The effects of protein removal
J Cell Biol.
1974 Dec.
Abstract
We have used freeze-etching and SDS-polyacrylamide gel electrophoresis to study the conditions under which the intramembrane particles of the human erythrocyte ghost may be aggregated. The fibrous membrane protein, spectrin, can be almost entirely removed from erythrocyte ghosts with little or no change in the distribution of the particles. However, after spectrin depletion, particle aggregation in the plane of the membrane may be induced by conditions which cause little aggregation in freshly prepared ghosts. This suggests that the spectrin molecules form a molecular meshwork which limits the translational mobility of the erythrocyte membrane particles.
Similar articles
-
Intramembrane particle aggregation in erythrocyte ghosts. II. The influence of spectrin aggregation.Biochim Biophys Acta. 1976 Feb 19;426(1):101-22. doi: 10.1016/0005-2736(76)90433-8. Biochim Biophys Acta. 1976. PMID: 2324
-
Erythrocyte membrane skeleton abnormalities in hereditary spherocytosis.Br J Haematol. 1983 Jun;54(2):173-87. doi: 10.1111/j.1365-2141.1983.tb02086.x. Br J Haematol. 1983. PMID: 6849841
-
Translational mobility of the membrane intercalated particles of human erythrocyte ghosts. pH-dependent, reversible aggregation.J Cell Biol. 1972 Jun;53(3):777-87. doi: 10.1083/jcb.53.3.777. J Cell Biol. 1972. PMID: 4554989 Free PMC article.
-
Red cell interactions in macromolecular suspension.Biorheology. 1979;16(3):137-48. doi: 10.3233/bir-1979-16302. Biorheology. 1979. PMID: 389308 Review. No abstract available.
-
Protein mobility in membranes.FEBS Lett. 1975 Jul 15;55(1):1-7. doi: 10.1016/0014-5793(75)80943-4. FEBS Lett. 1975. PMID: 237788 Review. No abstract available.
Cited by
-
Reconstitution of intramembrane particles in recombinants of erythrocyte protein band 3 and lipid: effects of spectrin-actin association.Proc Natl Acad Sci U S A. 1976 Nov;73(11):3891-5. doi: 10.1073/pnas.73.11.3891. Proc Natl Acad Sci U S A. 1976. PMID: 1069273 Free PMC article.
-
Induction of spectrin in erythroleukemic cells transformed by Friend virus.Proc Natl Acad Sci U S A. 1977 Sep;74(9):3898-902. doi: 10.1073/pnas.74.9.3898. Proc Natl Acad Sci U S A. 1977. PMID: 410028 Free PMC article.
-
Spectrin plus band 4.1 cross-link actin. Regulation by micromolar calcium.J Cell Biol. 1980 May;85(2):361-76. doi: 10.1083/jcb.85.2.361. J Cell Biol. 1980. PMID: 6892816 Free PMC article.
-
Spreading of wheat germ agglutinin-induced erythrocyte contact by formation of spatially discrete contacts.Cell Biophys. 1990 Jun;16(3):105-26. doi: 10.1007/BF02991425. Cell Biophys. 1990. PMID: 1698548
-
Direct visualization of the interrelationship between intramembrane and extracellular structures.Proc Natl Acad Sci U S A. 1989 Apr;86(8):2723-7. doi: 10.1073/pnas.86.8.2723. Proc Natl Acad Sci U S A. 1989. PMID: 2495534 Free PMC article.
