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. 1973 Mar;11(3):441-8.
doi: 10.1128/JVI.11.3.441-448.1973.

Isolation and properties of an RNA polymerase from influenza virus-infected cells

R W Compans, L A Caliguiri

Isolation and properties of an RNA polymerase from influenza virus-infected cells

R W Compans et al. J Virol. 1973 Mar.

Abstract

Structures with RNA polymerase activity were isolated from influenza virus-infected cells, and consisted of ribonucleoprotein (RNP) complexes, similar in morphology to the viral internal component or nucleocapsid. The isolation procedure involved fractionation of infected cells in a discontinuous sucrose gradient, in which enzyme activity was concentrated in a fraction of intermediate density which contains both smooth and rough cytoplasmic membranes. The RNPs with polymerase activity were further purified in a velocity gradient, after which the peak fractions showed a 35-fold purification of the polymerase activity when compared with cytoplasmic extracts. The NP polypeptide, which is the subunit of the virion RNP, was the only virus-specific polypeptide detected in these RNP structures.

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