Sequence-specific trans-activation of the adenovirus EIIa early promoter by the viral EIV transcription unit
- PMID: 4029123
- PMCID: PMC554377
- DOI: 10.1002/j.1460-2075.1985.tb03812.x
Sequence-specific trans-activation of the adenovirus EIIa early promoter by the viral EIV transcription unit
Abstract
The contribution of adenovirus early genes, other than that of the well-documented EIa immediate early gene, to the transcriptional regulation of the viral EIIa early transcription unit was examined. HeLa cells were transfected with EIIa-containing plasmids and co-transfected with distinct plasmids bearing one of the viral regions EIa, EIII or EIV. Co-transfection with the EIV-recombinants, but not the EIII constructs, stimulated specific transcription from the major EIIaE start site (EIIaE1) by 5- to 15-fold, as concluded from quantitative S1 nuclease analysis of cytoplasmic RNA and in vitro nuclear 'run-on' transcription assays. The extent of the EIV-induced stimulation was similar to that achieved by EIa under identical conditions. However, in contrast to our observations for EIa-mediated stimulation, where no unique EIIaE1 promoter elements were implicated, maximal induction by EIV requires sequences between positions -48 and -19 (with respect to the EIIaE1 start site).
Similar articles
-
Differential activation of the E2F transcription factor by the adenovirus EIa and EIV products in F9 cells.Proc Natl Acad Sci U S A. 1990 Mar;87(5):1782-6. doi: 10.1073/pnas.87.5.1782. Proc Natl Acad Sci U S A. 1990. PMID: 2137928 Free PMC article.
-
The adenovirus-2 early EIIa transcription unit possesses two overlapping promoters with different sequence requirements for EIa-dependent stimulation.EMBO J. 1985 May;4(5):1293-300. doi: 10.1002/j.1460-2075.1985.tb03775.x. EMBO J. 1985. PMID: 4006920 Free PMC article.
-
EIa-mediated stimulation of the adenovirus EIII promoter involves an enhancer element within the nearby EIIa promoter.J Virol. 1988 May;62(5):1762-7. doi: 10.1128/JVI.62.5.1762-1767.1988. J Virol. 1988. PMID: 2965764 Free PMC article.
-
Adenovirus EIIA early promoter: transcriptional control elements and induction by the viral pre-early EIA gene, which appears to be sequence independent.Proc Natl Acad Sci U S A. 1985 Apr;82(8):2230-4. doi: 10.1073/pnas.82.8.2230. Proc Natl Acad Sci U S A. 1985. PMID: 3857577 Free PMC article.
-
Individual products of the adenovirus 12S and 13S EIa mRNAs stimulate viral EIIa and EIII expression at the transcriptional level.Proc Natl Acad Sci U S A. 1984 Jul;81(14):4381-5. doi: 10.1073/pnas.81.14.4381. Proc Natl Acad Sci U S A. 1984. PMID: 6336330 Free PMC article.
Cited by
-
E1A-mediated activation of the adenovirus E4 promoter can occur independently of the cellular transcription factor E4F.Mol Cell Biol. 1991 Sep;11(9):4297-305. doi: 10.1128/mcb.11.9.4297-4305.1991. Mol Cell Biol. 1991. PMID: 1831536 Free PMC article.
-
Biosynthesis and properties of the adenovirus 2 L1-encoded 52,000- and 55,000-Mr proteins.J Virol. 1986 Mar;57(3):839-47. doi: 10.1128/JVI.57.3.839-847.1986. J Virol. 1986. PMID: 3005630 Free PMC article.
-
The adenovirus E4 gene, in addition to the E1A gene, is important for trans-activation of E2 transcription and for E2F activation.J Virol. 1989 Sep;63(9):3643-50. doi: 10.1128/JVI.63.9.3643-3650.1989. J Virol. 1989. PMID: 2527310 Free PMC article.
-
The adenovirus E4 17-kilodalton protein complexes with the cellular transcription factor E2F, altering its DNA-binding properties and stimulating E1A-independent accumulation of E2 mRNA.J Virol. 1990 May;64(5):2345-59. doi: 10.1128/JVI.64.5.2345-2359.1990. J Virol. 1990. PMID: 2139141 Free PMC article.
-
Differential activation of the E2F transcription factor by the adenovirus EIa and EIV products in F9 cells.Proc Natl Acad Sci U S A. 1990 Mar;87(5):1782-6. doi: 10.1073/pnas.87.5.1782. Proc Natl Acad Sci U S A. 1990. PMID: 2137928 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
