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. 2025:2858:113-122.
doi: 10.1007/978-1-0716-4140-8_10.

Human Corneal Rim Perfusion Culture

Weiming Mao  1
Affiliations

Human Corneal Rim Perfusion Culture

Weiming Mao. Methods Mol Biol. 2025.

Abstract

Human anterior segment perfusion cultures are frequently used for trabecular meshwork research. However, this model requires the use of whole eye globes which are expensive. Here, we describe a method using human corneal rims as an alternative to anterior segments for perfusion culture. In this method, the human corneal rim is glued to a three-dimensional-printed perfusion plates instead of using mechanical compression in traditional perfusion culture setup. The corneal rim and perfusion plate are placed onto a special holder for perfusion culture. This method is affordable and easy to set up. The instruments for traditional perfusion culture studies can be used for this model without modification.

Keywords: Corneal rim perfusion culture; Intraocular pressure; Outflow facility.

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