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. 2024 Oct 15:79:100402.
doi: 10.1016/j.clinsp.2024.100402. eCollection 2024.

Changes in PEDF, MMP-2, and TGF-β2 levels in the aqueous humor of cataract patients and their correlation with disease severity

Yong Feng Lin  1 , Jin Xia Xie  2 , Xiao Luan Chen  3
Affiliations

Changes in PEDF, MMP-2, and TGF-β2 levels in the aqueous humor of cataract patients and their correlation with disease severity

Yong Feng Lin et al. Clinics (Sao Paulo). .

Abstract

Objective: To explore the changes of Pigment Epithelium-Derived Factor (PEDF), Matrix Metalloproteinase-2 (MMP-2), and Transforming Growth Factor-β2 (TGF-β2) levels in the aqueous humor of cataract patients and their correlation with disease severity.

Methods: 93 cataract patients and 56 healthy subjects were study objects. PEDF, MMP-2, and TGF-β2 levels of aqueous humor were compared, and the correlation between each index and Lens Opacity Classification System (LOCS) III classification was analyzed. ROC curve was used to analyze the evaluation value of the combined detection of each index on cataract development, and logistic regression to analyze the influence of the changes of each index on cataract development.

Results: PEDF levels were lower and MMP-2 and TGF-β2 levels were higher in the aqueous humor of cataract patients than in healthy subjects. PEDF levels in the aqueous humor were negatively correlated with LOCS III classification, while MMP-2 and TGF-β2 levels were positively correlated with LOCS III classification. The AUC value of combined detection was higher than that of PEDF, MMP-2, and TGF-β2 in the aqueous humor alone. MMP-2 ≥ 15.13 pg/mL, TGF-β2 ≥ 385.91 pg/mL and PEDF < 198.85 ng/mL were risk factors for cataract development.

Conclusion: The changes in PEDF, MMP-2, and TGF-β2 levels in the aqueous humor of cataract patients are related to LOCS III classification. The combined detection is valuable in evaluating cataract development.

Keywords: Aqueous Humor; Cataract; LOCS III Classification; Matrix Metalloproteinase-2; Pigment Epithelial-Derived Factor; Transforming Growth Factor-β2.

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Conflict of interest statement

Declaration of competing interest The authors declare no conflicts of interest.

Figures

Fig 1
Fig. 1
PEDF, MMP-2, and TGF-β2 levels in cataract patients and healthy subjects (*p < 0.05 vs. control group).
Fig 2
Fig. 2
PEDF, MMP-2, and TGF-β2 levels in the aqueous humor of patients with different LOCS III classification.
Fig 3
Fig. 3
ROC curve of PEDF, MMP-2, and TGF-β2 levels in the aqueous humor.
See this image and copyright information in PMC

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