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. 2024 Dec;31(12):3768-3778.
doi: 10.1007/s43032-024-01703-8. Epub 2024 Oct 15.

Characterizing the Impact of Dysregulated Micrornas on CRISP3 Isoforms in Male Infertility

Delnya Gholami  1   2 , Farzane Amirmahani  3 , Reza Salman Yazdi  4 , Maryam Nemati-Dehkordi  5 , Hossein Teimori  6
Affiliations

Characterizing the Impact of Dysregulated Micrornas on CRISP3 Isoforms in Male Infertility

Delnya Gholami et al. Reprod Sci. 2024 Dec.

Abstract

microRNAs (miRNAs) have a serious and dynamic function in spermatogenesis. These molecules have been recognized as crucial parts of the control of gene activity, and their involvement in the regulation of target genes has been extensively studied. This research aimed to determine the expression of CRISP3 and miR-493-5p, miR-204-5p, and miR-182-5p in the seminal plasma fluid and spermatozoa and to examine the relationship between CRISP3 and the mentioned miRNAs in 57 infertile men with Asthenozoospermia (AZ) (n = 19), Teratoasthenozoospermia (TAZ) (n = 19), and Normozoospermia (NZ) (n = 19). The selection of these three miRNAs, miR-493-5p, miR-204-5p, and miR-182-5p, was conducted using computational prediction algorithms. These miRNAs were nominated as CRISP3-associated miRNAs that can target CRISP3. We performed the quantitative real-time polymerase chain reaction (qRT-PCR) method to determine the levels of the studied miRNA expression. In the following stage, the expression of two protein isoforms of CRISP3, targeted by these miRNAs, was quantified using western blotting. The results demonstrate significant differences in the levels of miR-182-5p, miR-204-5p, miR-493-5p, and CRISP3 isoforms among the patient groups. In TAZ individuals, miR-182-5p and miR-204-5p expression decreased, while miR-493-5p expression increased compared to the control samples. Additionally, significant differences were observed in the expression levels of unglycosylated and glycosylated CRISP3 isoforms between the AZ and NZ groups. Correlation analysis revealed associations between miRNA expression and the expression of CRISP3 isoforms in the patient groups. Additionally, there were correlations between the expression of CRISP3 isoforms and sperm motility and morphology. These results offer valuable insights into the underlying molecular processes associated with male infertility.

Keywords: Asthenozoospermia; CRISP3; Male infertility; MiRNAs; Morphology; Sperm motility; Teratoasthenozoospermia.

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Conflict of interest statement

Declarations. IRB Approval: All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. This study was approved by the ethics committee of Shahrekord University of Medical Sciences (ethics code: IR.SKUMS.REC.1397.172). Competing Interest: All authors declare no potential competing interest.

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References

    1. Abu-Halima M, Backes C, Leidinger P, Keller A, Lubbad AM, Hammadeh M, Meese E. MicroRNA expression profiles in human testicular tissues of infertile men with different histopathologic patterns. Fertil Steril. 2014;101(1):78-86.e72. - DOI - PubMed
    1. Abu-Halima M, Becker LS, Ayesh BM, Meese E. MicroRNA-targeting in male infertility: sperm microRNA-19a/b-3p and its spermatogenesis related transcripts content in men with oligoasthenozoospermia. Front Cell Dev Biol. 2022;10. https://doi.org/10.3389/fcell.2022.973849 .
    1. Abu-Halima M, Hammadeh M, Schmitt J, Leidinger P, Keller A, Meese E, Backes C. Altered microRNA expression profiles of human spermatozoa in patients with different spermatogenic impairments. Fertil Steril. 2013;99(5):1249–1255. e1216.
    1. Amirmahani F, Asadi MH, Jannat Alipoor F. LncRNA MIAT Promotes the proliferation and invasion of colorectal cancer via suppressing apoptosis and senescence. Middle East J Cancer. 2023;14(2):219–29.
    1. Amirmahani F, Ebrahimi N, Askandar RH, RasouliEshkaftaki M, Fazeli K, Hamblin MR. "Long Noncoding RNAs CAT2064 and CAT2042 may Function as Diagnostic Biomarkers for Prostate Cancer by Affecting Target MicrorRNAs. Indian J Clin Biochem. 2021;39(3):322–30. - DOI - PubMed

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