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Review
. 2024 Nov;29(11):104196.
doi: 10.1016/j.drudis.2024.104196. Epub 2024 Oct 4.

3D light-sheet fluorescence microscopy in preclinical and clinical drug discovery

Urmas Roostalu  1 , Henrik H Hansen  2 , Jacob Hecksher-Sørensen  2
Affiliations
Review

3D light-sheet fluorescence microscopy in preclinical and clinical drug discovery

Urmas Roostalu et al. Drug Discov Today. 2024 Nov.

Abstract

Light-sheet fluorescence microscopy (LSFM) combined with tissue clearing has emerged as a powerful technology in drug discovery. LSFM is applicable to a variety of samples, from rodent organs to clinical tissue biopsies, and has been used for characterizing drug targets in tissues, demonstrating the biodistribution of pharmaceuticals and determining their efficacy and mode of action. LSFM is scalable to high-throughput analysis and provides resolution down to the single cell level. In this review, we describe the advantages of implementing LSFM into the drug discovery pipeline and highlight recent advances in this field.

Keywords: biodistribution imaging; light sheet fluorescence microscopy; neurodegenerative diseases; neuronal activation; preclinical imaging; preclinical tumor imaging; vasculature imaging.

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