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. 1985 May 17;228(4701):810-5.
doi: 10.1126/science.3923623.

Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins

G G Wong, J S Witek, P A Temple, K M Wilkens, A C Leary, D P Luxenberg, S S Jones, E L Brown, R M Kay, E C Orr, et al.

Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins

G G Wong et al. Science. .

Abstract

Clones of complementary DNA encoding the human lymphokine known as granulocyte-macrophage colony-stimulating factor (GM-CSF) were isolated by means of a mammalian cell (monkey COS cell) expression screening system. One of these clones was used to produce recombinant GM-CSF in mammalian cells. The recombinant hematopoietin was similar to the natural product that was purified to apparent homogeneity from medium conditioned by a human T-cell line. The human T-cell GM-CSF was found to be 60 percent homologous with the GM-CSF recently cloned from murine lung messenger RNA.

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