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. 2024 Aug 2;13(8):649.
doi: 10.3390/pathogens13080649.

Postmortem Sampling in Piglet Populations: Unveiling Specimens Accuracy for Porcine Reproductive and Respiratory Syndrome Detection

Mariana Kikuti  1 , Claudio Marcello Melini  1 , Xiaomei Yue  1 , Marie Culhane  1 , Cesar A Corzo  1
Affiliations

Postmortem Sampling in Piglet Populations: Unveiling Specimens Accuracy for Porcine Reproductive and Respiratory Syndrome Detection

Mariana Kikuti et al. Pathogens. .

Abstract

Specimens collected from dead pigs are a welfare-friendly and cost-effective active surveillance. This study aimed to evaluate the accuracy of different postmortem specimens from dead piglets for disease detection, using PRRSV as an example. Three farrow-to-wean farms undergoing PRRSV elimination were conveniently selected. Samples were collected at approximately 8- and 20-weeks post-outbreak. Postmortem specimens included nasal (NS), oral (OS), and rectal (RS) swabs, tongue-tip fluids (TTF), superficial inguinal lymph nodes (SIL), and intracardiac blood. These were tested individually for PRRSV by RT-PCR. Sensitivity, specificity, negative and positive predictive values, and agreement of postmortem specimens were calculated using intracardiac sera as the gold standard. OS and SIL had the best overall performance, with sensitivities of 94.6-100%, specificities of 83.9-85.1%, and negative predictive values of 97.3-100%. TTF had high sensitivity (92.2%) but low specificity (53.9%) and positive predictive value (48.3%). While challenges in meeting sampling targets due to variable pre-weaning mortality were noted, PRRS was detected in all postmortem specimens. OS and NS showed promising results for disease monitoring, though TTF, despite their sensitivity, had lower specificity, making them less suitable for individual infection assessment but useful for assessing environmental contamination.

Keywords: PRRSV detection; diagnostic accuracy; disease monitoring; postmortem sampling; specimen sensitivity.

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Conflict of interest statement

The authors declare no conflicts of interest.

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References

    1. Corzo C.A., Mondaca E., Wayne S., Torremorell M., Dee S., Davies P., Morrison R.B. Control and elimination of porcine reproductive and respiratory syndrome virus. Virus Res. 2010;154:185–192. doi: 10.1016/j.virusres.2010.08.016. - DOI - PubMed
    1. Linhares D.C.L., Cano J.P., Torremorell M., Morrison R.B. Comparison of time to PRRSv-stability and production losses between two exposure programs to control PRRSv in sow herds. Prev. Vet. Med. 2014;116:111–119. doi: 10.1016/j.prevetmed.2014.05.010. - DOI - PubMed
    1. Neumann E.J., Kliebenstein J.B., Johnson C.D., Mabry J.W., Bush E.J., Seitzinger A.H., Green A.L., Zimmerman J.J. Assessment of the economic impact of porcine reproductive and respiratory syndrome on swine production in the United States. J. Am. Vet. Med. Assoc. 2005;227:385–392. doi: 10.2460/javma.2005.227.385. - DOI - PubMed
    1. Holtkamp D., Kliebenstein J., Neumann E., Zimmerman J., Rotto H., Yoder T., Wang C., Yeske P., Mowrer C., Haley C. Assessment of the economic impact of porcine reproductive and respiratory syndrome virus on United States pork producers. J. Swine Health Prod. 2013;21:72–84. doi: 10.54846/jshap/754. - DOI - PubMed
    1. Morrison Swine Health Monitoring Project PRRS Prevalence of Sow Herd Status Beginning 1 July 2009. [(accessed on 27 July 2024)]. Available online: https://mshmp.umn.edu/reports#Charts.

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