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. 2024 Aug 7;12(8):1609.
doi: 10.3390/microorganisms12081609.

The Effects of Aspirin Intervention on Inflammation-Associated Lingual Bacteria: A Pilot Study from a Randomized Clinical Trial

Guillaume C Onyeaghala  1 , Shweta Sharma  2 , Mosunmoluwa Oyenuga  3 , Christopher M Staley  4   5 , Ginger L Milne  6 , Ryan T Demmer  7 , Aasma Shaukat  8 , Bharat Thyagarajan  2   9 , Robert J Straka  10 , Timothy R Church  5   11 , Anna E Prizment  2   9
Affiliations

The Effects of Aspirin Intervention on Inflammation-Associated Lingual Bacteria: A Pilot Study from a Randomized Clinical Trial

Guillaume C Onyeaghala et al. Microorganisms. .

Abstract

Several bacterial taxa enriched in inflammatory bowel diseases and colorectal cancer (CRC) are found in the oral cavity. We conducted a pilot study nested within a six-week aspirin intervention in a randomized placebo-controlled trial to test their response to aspirin intervention. Fifty healthy subjects, 50-75 years old, were randomized to receive 325 mg aspirin (n = 30) or placebo (n = 20) orally once daily for six weeks. Oral tongue swabs were collected at baseline and week six. We estimated the association between aspirin use and the temporal changes in the relative abundance of pre-specified genus level taxa from pre- to post-treatment. The temporal change in relative abundance differed for eight genus level taxa between the aspirin and placebo groups. In the aspirin group, there were significant increases in the relative abundances of Neisseria, Streptococcus, Actinomyces, and Rothia and significant decreases in Prevotella, Veillonella, Fusobacterium, and Porphyromonas relative to placebo. The log ratio of Neisseria to Fusobacterium declined more in the aspirin group than placebo, signaling a potential marker associated with aspirin intervention. These preliminary findings should be validated using metagenomic sequencing and may guide future studies on the role of aspirin on taxa in various oral ecological niches.

Keywords: aspirin; colorectal cancer; oral microbiome; periodontitis.

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Conflict of interest statement

There are no competing financial or non-financial interests in relation to this manuscript. The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
ASMIC trial and intervention CONSORT diagram.
Figure 2
Figure 2
Distribution of pre-specified oral taxa (genus level) in the ASMIC trial. Prevalence represents the detection prevalence (presence vs. absence of taxa across samples, %). Abundance represents the relative abundance (i.e., the presence of a particular organism compared to all other organisms in a given sample, %). * The Prevotella genus level was reported as “Prevotella_D_7” by QIIME2 version 2023.10.
Figure 3
Figure 3
Change in relative abundance of pre-specified taxa in the aspirin and placebo groups from baseline (week 0) to post-intervention (week 6).
Figure 4
Figure 4
Change in the balance in Neisseria to Fusobacterium (at the family level) in the aspirin and placebo group from baseline (week 0) to post-intervention (week 6).
See this image and copyright information in PMC

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