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. 2024 Aug 22.
doi: 10.1007/s12033-024-01250-2. Online ahead of print.

ALKBH5-Mediated m6A Modification Drives Apoptosis in Renal Tubular Epithelial Cells by Negatively Regulating MUC1

Wenwei Chen #  1   2   3 , Changyi Liu #  1   2   3 , Yanfeng He  1   2   3 , Tao Jiang  1   2   3 , Qin Chen  1   2   3 , Hua Zhang  1   2   3 , Rui Gao  4   5   6
Affiliations

ALKBH5-Mediated m6A Modification Drives Apoptosis in Renal Tubular Epithelial Cells by Negatively Regulating MUC1

Wenwei Chen et al. Mol Biotechnol. .

Abstract

Dysregulation of renal tubular epithelial cell (RTEC) apoptosis is one of the critical steps underlying the occurrence and development of nephrolithiasis. Although N6-methyladenosine (m6A) modification has been extensively studied and associated with various pathologic processes, research on its specific role in RTEC injury and apoptosis remains limited. In this study, we found that overexpression of ALKBH5 reduced the level of m6A modification in RTEC cells and notably promoted RTEC apoptosis. Further mechanism studies revealed that ALKBH5 mainly decreased the m6A level on the mRNA of Mucin 1 (MUC1) gene in RTECs. Moreover, ALKBH5 impaired the stability of MUC1 mRNA in RTECs, leading to attenuated expression of MUC1. Finally, we determined that the ALKBH5-MUC1 axis primarily facilitated RTEC apoptosis by regulating the PI3K/Akt signaling pathway. This study revealed the critical role of the ALKBH5-MUC1-PI3K/Akt regulatory system in RTEC apoptosis and provided new therapeutic targets for treating nephrolithiasis.

Keywords: ALKBH5; Apoptosis; MUC1; N6-methyladenosine; Nephrolithiasis; PI3K/Akt.

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References

    1. Singh, P., Harris, P. C., Sas, D. J., & Lieske, J. C. (2022). The genetics of kidney stone disease and nephrocalcinosis. Nature Reviews Nephrology, 18, 224–240. - PubMed - DOI
    1. Luft, F. C. (2021). Biomarkers and predicting acute kidney injury. Acta Psychologica, 231, e13479.
    1. Keddis, M. T., & Rule, A. D. (2013). Nephrolithiasis and loss of kidney function. Current Opinion in Nephrology and Hypertension, 22, 390–396. - PubMed - PMC - DOI
    1. Khan, S. R., Pearle, M. S., Robertson, W. G., Gambaro, G., Canales, B. K., Doizi, S., Traxer, O., & Tiselius, H. G. (2016). Kidney stones. Nature Reviews Disease Primers, 2, 16008. - PubMed - PMC - DOI
    1. Mrowka, R. (2024). Recent advances in kidney research. Acta Physiologica (Oxford)., 235, e14144. - DOI

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