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. 2024 Aug 20;74(3):79.
doi: 10.1007/s12031-024-02256-w.

Electroacupuncture Alleviates Streptozotocin-Induced Diabetic Neuropathic Pain via the TRPV1-Mediated CaMKII/CREB Pathway in Rats

Yinmu Zheng #  1   2 , Siyi Li #  1   2 , Yurong Kang  1   2 , Qunqi Hu  1   2 , Yu Zheng  1   2 , Xiaoxiang Wang  2 , Hengyu Chi  2 , Keying Guo  2 , Minjian Jiang  1   2 , Zhouyuan Wei  1   2 , Xiaomei Shao  1   2 , Chi Xu  1   2 , Boyu Liu  1   2 , Junying Du  1   2 , Xiaofen He  1   2 , Jianqiao Fang  1   2 , Zhenzhong Lu  3 , Yongliang Jiang  4   5
Affiliations

Electroacupuncture Alleviates Streptozotocin-Induced Diabetic Neuropathic Pain via the TRPV1-Mediated CaMKII/CREB Pathway in Rats

Yinmu Zheng et al. J Mol Neurosci. .

Abstract

Diabetic neuropathic pain (DNP) is a diabetic complication that causes severe pain and deeply impacts the quality of the sufferer's daily life. Currently, contemporary clinical treatments for DNP generally exhibit a deficiency in effectiveness. Electroacupuncture (EA) is recognized as a highly effective and safe treatment for DNP with few side effects. Regrettably, the processes via which EA alleviates DNP are still poorly characterized. Transient receptor potential vanilloid 1 (TRPV1) and phosphorylated calcium/calmodulin-dependent protein kinase II (p-CaMKII) are overexpressed on spinal cord dorsal horn (SCDH) in DNP rats, and co-localization is observed between them. Capsazepine, a TRPV1 antagonist, effectively reduced nociceptive hypersensitivity and downregulated the overexpression of phosphorylated CaMKIIα in rats with DNP. Conversely, the CaMKII inhibitor KN-93 did not have any impact on TRPV1. EA alleviated heightened sensitivity to pain caused by nociceptive stimuli and downregulated the level of TRPV1, p-CaMKIIα, and phosphorylated cyclic adenosine monophosphate response element-binding protein (p-CREB) in DNP rats. Intrathecal injection of capsaicin, on the other hand, reversed the above effects of EA. These findings indicated that the CaMKII/CREB pathway on SCDH is located downstream of TRPV1 and is affected by TRPV1. EA alleviates DNP through the TRPV1-mediated CaMKII/CREB pathway.

Keywords: CaMKII/CREB pathway; Capsaicin; Capsazepine; Diabetic neuropathic pain; KN-93; TRPV1.

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