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. 2024 Aug;23(4):896-908.
doi: 10.1007/s12663-024-02250-0. Epub 2024 Jun 16.

Unique Cohorts of Salivary Gland Cancer Cells as an in-vitro Model of Circulating Tumor Cells

Callie Mincy  1   2 , Luke Revelt  1 , Kathryn Carter  1 , Donald Reed  1 , Anita Joy  3
Affiliations

Unique Cohorts of Salivary Gland Cancer Cells as an in-vitro Model of Circulating Tumor Cells

Callie Mincy et al. J Maxillofac Oral Surg. 2024 Aug.

Abstract

Introduction: The characterization of circulating tumor cells (CTC) and circulating tumor microemboli (CTM) has emerged as both a challenge to the standard view of metastasis, and as a valuable means for understanding genotypic and phenotypic variability shown even within the same cancer type. However, in the case of salivary gland neoplasms, limited data are available for the role that CTCs and CTMs play in metastasis and secondary tumor formation.ru.AQ1 In response to this, we propose that similarities between in vitro clusters of cultured salivary gland cancer cells may act as a surrogate model for in vivo CTCs and CTMs isolated from patients.

Materials and methods: Using techniques in immunofluorescence, immunoblotting, and 2-dimensional migration, we isolated and characterized a group of cohort cells from a commercially available cell line (HTB-41). Results: Here, cells exhibited a hybrid phenotype with simultaneous expression of both epithelial and mesenchymal markers (E-cadherin, vimentin, and α-SMA). Cohort cells also exhibited increased migration in comparison to parental cells.

Conclusion: Data suggest that these isolated cell clusters may fucntion as a potential in vitro model of CTCs and CTMs.

Keywords: Circulating tumor cells; Circulating tumor microemboli; E-cadherin; Epithelial to mesenchymal transition; Metastasis; Salivary gland cancer; Vimentin; α-SMA; β- catenin.

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