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. 2024 Jan;12(4):631-642.
doi: 10.61186/rbmb.12.4.631.

Correlation of MicroRNA-125b, Sirtuin, and Signal Transducer and Activator of Transcription 3 with Biochemical Parameters and Risk Factors in Atherosclerosis Patients

Atefeh Mirderikvand  1 , Gholamreza Shahsavari  1 , Alireza Moayyed Kazemi  2 , Fatemeh Ahmadpour  3 , Banafsheh Yalameha  4   5
Affiliations

Correlation of MicroRNA-125b, Sirtuin, and Signal Transducer and Activator of Transcription 3 with Biochemical Parameters and Risk Factors in Atherosclerosis Patients

Atefeh Mirderikvand et al. Rep Biochem Mol Biol. 2024 Jan.

Abstract

Background: Atherosclerosis (AS) is an inflammatory disease linked to vascular events, with dysregulation of microRNA (miR)-125b, contributing to cardiovascular disease pathogenesis. Moreover, there is evidence of the involvement of signal transducer and activator of transcription 3 (STAT3) and sirtuin 6 (SIRT6) in AS. This study aimed to survey the expression levels of miR-125b, STAT3, and SIRT6 in the peripheral blood mononuclear cells (PBMCs) of AS patients and controls, and to find their correlations with biochemical parameters and risk factors.

Methods: This study included blood samples from 45 controls and 45 AS patients, with PBMCs isolated using Ficoll solution. Expression levels of miR-125b, STAT3, and SIRT6 were determined via quantitative Real Time-PCR.

Results: The findings revealed a significant increase in miR-125b levels in patients compared to controls (P = 0.017). However, alterations in STAT3 and SIRT6 expression were not significant (P> 0.05). There was no substantial relationship between miR-125b and STAT3 (P = 0.522) or SIRT6 (P = 0.88). miR-125b showed a significant relationship with atherogenic indexes and creatinine (P<0.05), while the association of SIRT6 with HDL and creatinine was significant (P<0.05). STAT3 exhibited high diagnostic power for identifying individuals at risk of heart disease and hypertension (P<0.05).

Conclusion: STAT3 can serve as a valuable biomarker for detecting AS and AS-related risk factors. miR-125b and SIRT6 may be associated with AS lipid metabolism. However, further studies with larger sample sizes are recommended to mechanistically elucidate the association of these genes.

Keywords: Atherosclerosis; Leukocyte; SIRT6; STAT3; microRNA-125b.

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Conflict of interest statement

The authors declare that they have no conflict of interest

Figures

Fig. 1
Fig. 1
Expression levels of (a) miR-125b; (b) SIRT6, and (c) STAT3 in patient and control groups. *P<0.05.
Fig. 2
Fig. 2
ROC curves of STAT3 diagnostic value to detect individuals at risk (a) Heart disease; (b) Hypertension.
Fig. 3
Fig. 3
Correlation between miR-125b and SIRT6 gene with biochemical parameters. (a) Correlation of miR-125b with AIP; (b) Correlation of miR-125b with Cr; (c) Correlation of SIRT6 with HDL; (d) Correlation of SIRT6 with Cr. AIP, Atherogenic index of plasma; Cr, Creatinine; HDL, High-density lipoprotein. *P<0.05.
Fig. 4
Fig. 4
The expression levels of STAT3 in PBMC cells. (a) Groups with a history of heart disease and without a history of heart disease; (b) Groups with hypertension and without hypertension. * P<0.05.
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