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Review
. 2024 Jul 2;7(4):52.
doi: 10.3390/mps7040052.

Methods to Investigate the Secretome of Senescent Cells

Afshin Samiminemati  1 , Domenico Aprile  1 , Dario Siniscalco  1 , Giovanni Di Bernardo  1   2
Affiliations
Review

Methods to Investigate the Secretome of Senescent Cells

Afshin Samiminemati et al. Methods Protoc. .

Abstract

The word "secretome" was first used to describe the proteins that cells secrete under different circumstances; however, recent studies have proven the existence of other molecules such as RNA and chemical compounds in the secretome. The study of secretome has significance for the diagnosis and treatment of disease as it provides insight into cellular functions, including immune responses, development, and homeostasis. By halting cell division, cellular senescence plays a role in both cancer defense and aging by secreting substances known as senescence-associated secretory phenotypes (SASP). A variety of techniques could be used to analyze the secretome: protein-based approaches like mass spectrometry and protein microarrays, nucleic acid-based methods like RNA sequencing, microarrays, and in silico prediction. Each method offers unique advantages and limitations in characterizing secreted molecules. Top-down and bottom-up strategies for thorough secretome analysis are became possible by mass spectrometry. Understanding cellular function, disease causes, and proper treatment targets is aided by these methodologies. Their approaches, benefits, and drawbacks will all be discussed in this review.

Keywords: RNA sequencing; SASP; secretome; senescence; spectrometry.

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Conflict of interest statement

The authors declare no conflicts of interest.

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Grants and funding

This research was funded by MUR PRIN 2022, “Is it possible to improve the success rate of cellular therapy based on Mesenchymal Stromal cells?”, CUP B53D23011280006 grant to G.D.B.

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