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. 2024 Jul 5;13(13):2146.
doi: 10.3390/foods13132146.

Influence of Oleacein, an Olive Oil and Olive Mill Wastewater Phenolic Compound, on Caenorhabditis elegans Longevity and Stress Resistance

Morgane Carrara  1 , Myriam Richaud  2 , Pierre Cuq  2 , Simon Galas  2 , Delphine Margout-Jantac  1
Affiliations

Influence of Oleacein, an Olive Oil and Olive Mill Wastewater Phenolic Compound, on Caenorhabditis elegans Longevity and Stress Resistance

Morgane Carrara et al. Foods. .

Abstract

Oleacein, a bioactive compound of olive oil and olive mill wastewater, has one of the strongest antioxidant activities among olive phenolics. However, few reports explore the in vivo antioxidant activity of oleacein, with no clear identification of the biological pathway involved. Earlier studies have demonstrated a link between stress resistance, such as oxidative stress, and longevity. This study presents the effects of oleacein on Caenorhabditis elegans mean lifespan and stress resistance. A significant lifespan extension was observed with an increase of 20% mean lifespan at 5 µg/mL with a hormetic-like dose-dependent effect. DAF-16 and SIR-2.1 were involved in the effects of oleacein on the longevity of C. elegans, while the DAF-2 receptor was not involved. This study also shows the capacity of oleacein to significantly enhance C. elegans resistance to oxidative and thermal stress and allows a better understanding of the positive effects of olive phenolics on health.

Keywords: C. elegans; aging; oleacein; olive mill wastewater; olive oil.

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Conflict of interest statement

The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

Figures

Figure 1
Figure 1
Oleacein structure.
Figure 2
Figure 2
Oleacein stability in S-base at 20 °C.
Figure 3
Figure 3
Oleacein impact on wild-type (N2) C. elegans lifespan.
Figure 4
Figure 4
Comparative effects of oleacein and hydroxytyrosol treatment on wild-type C. elegans lifespan.
Figure 5
Figure 5
Effect of oleacein on C. elegans mutants’ lifespan.
Figure 6
Figure 6
Effect of oleacein on DAF-16::GFP cellular localization.
Figure 7
Figure 7
Effect of oleacein on wild-type C. elegans thermal (A) and oxidative stress (B) resistance.
Figure 8
Figure 8
Possible mechanisms of action of oleacein.
See this image and copyright information in PMC

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