doi: 10.1016/0006-291x(85)91784-x.
Characteristics of hepatitis B surface antigen produced in yeast
- PMID: 3899110
- DOI: 10.1016/0006-291x(85)91784-x
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Characteristics of hepatitis B surface antigen produced in yeast
Biochem Biophys Res Commun.
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Abstract
We have constructed an expression plasmid for regulated expression of the hepatitis B surface antigen gene in yeast using promoter of the yeast Pho5 gene. In the yeast transformants, the monomeric HBsAg (22K dalton) was estimated to constitute approximately 3% of the total proteins. On extraction, the HBsAg was found to have a buoyant density of 1.18 g/ml and an Sw.20 value of 54. Electron microscopy revealed particles of heterogeneous size ranging from 18-28 nm. When the yeast HBsAg was used to immunize guinea pigs, the anti-HBsAg antibodies produced could react with human serum HBsAg.
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