Functional Analysis of Seed Dormancy Genes by Biolistic Transient Gene Expression in Immature Embryos of Wheat

doi:10.1007/978-1-0716-3965-8_12

. 2024:2830:131-136.

doi: 10.1007/978-1-0716-3965-8_12.

Functional Analysis of Seed Dormancy Genes by Biolistic Transient Gene Expression in Immature Embryos of Wheat

Fumitaka Abe¹, Masahiko Mori^{1

2}, Shingo Nakamura³

Affiliations

¹ Institute of Crop Science, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan.
² Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
³ Institute of Crop Science, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan. shingo@affrc.go.jp.

PMID: 38977574
DOI: 10.1007/978-1-0716-3965-8_12

Functional Analysis of Seed Dormancy Genes by Biolistic Transient Gene Expression in Immature Embryos of Wheat

Fumitaka Abe et al. Methods Mol Biol. 2024.

. 2024:2830:131-136.

doi: 10.1007/978-1-0716-3965-8_12.

Authors

Fumitaka Abe¹, Masahiko Mori^{1

2}, Shingo Nakamura³

Affiliations

¹ Institute of Crop Science, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan.
² Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
³ Institute of Crop Science, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan. shingo@affrc.go.jp.

PMID: 38977574
DOI: 10.1007/978-1-0716-3965-8_12

Abstract

Seed dormancy genes typically suppress germination and cell division. Therefore, overexpressing these genes can negatively affect tissue culture, interfering with the generation of transgenic plants and thus hampering the analysis of gene function. Transient expression in target cells is a useful approach for studying the function of seed dormancy genes. Here, we describe a protocol for transiently expressing genes related to seed dormancy in the scutellum of immature wheat (Triticum aestivum) embryos to analyze their effects on germination.

Keywords: Biolistic delivery; Immature embryo; Mother of FT and TFL1; Precocious germination; Tissue culture; Transient gene expression; Wheat.

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References

1. Hensel G (2020) Genetic transformation of Triticeae cereals – Summary of almost three-decade’s development. Biotechnol Adv 40:107484 - DOI - PubMed
1. Raz V, Bergervoet JHW, Koornneef M (2001) Sequential steps for developmental arrest in Arabidopsis seeds. Development 128:243–252 - DOI - PubMed
1. Ashikawa I, Mori M, Nakamura S, Abe F (2014) A transgenic approach to controlling wheat seed dormancy level by using Triticeae DOG1-like genes. Transgenic Res 23:621–629 - DOI - PubMed
1. Nakamura S, Abe F, Kawahigashi H, Nakazono K, Tagiri A, Matsumoto T, Utsugi S, Ogawa T, Handa H, Ishida H, Mori M, Kawaura K, Ogihara Y, Miura H (2011) A Wheat Homolog of MOTHER OF FT AND TFL1 Acts in the Regulation of Germination. Plant Cell 23:3215–3229 - DOI - PubMed - PMC
1. Pellegrineschi A, Noguera LM, Skovmand B, Brito RM, Velazquez L, Salgado MM, Hrnandez R, Warburton M, Hoisington D (2002) Identification of highly transformable wheat genotypes for mass production of fertile transgenic plants. Genome 45:421–430 - DOI - PubMed

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[1] Hensel G (2020) Genetic transformation of Triticeae cereals – Summary of almost three-decade’s development. Biotechnol Adv 40:107484 - DOI - PubMed

[2] Hensel G (2020) Genetic transformation of Triticeae cereals – Summary of almost three-decade’s development. Biotechnol Adv 40:107484 - DOI - PubMed

[3] Raz V, Bergervoet JHW, Koornneef M (2001) Sequential steps for developmental arrest in Arabidopsis seeds. Development 128:243–252 - DOI - PubMed

[4] Raz V, Bergervoet JHW, Koornneef M (2001) Sequential steps for developmental arrest in Arabidopsis seeds. Development 128:243–252 - DOI - PubMed

[5] Ashikawa I, Mori M, Nakamura S, Abe F (2014) A transgenic approach to controlling wheat seed dormancy level by using Triticeae DOG1-like genes. Transgenic Res 23:621–629 - DOI - PubMed

[6] Ashikawa I, Mori M, Nakamura S, Abe F (2014) A transgenic approach to controlling wheat seed dormancy level by using Triticeae DOG1-like genes. Transgenic Res 23:621–629 - DOI - PubMed

[7] Nakamura S, Abe F, Kawahigashi H, Nakazono K, Tagiri A, Matsumoto T, Utsugi S, Ogawa T, Handa H, Ishida H, Mori M, Kawaura K, Ogihara Y, Miura H (2011) A Wheat Homolog of MOTHER OF FT AND TFL1 Acts in the Regulation of Germination. Plant Cell 23:3215–3229 - DOI - PubMed - PMC

[8] Nakamura S, Abe F, Kawahigashi H, Nakazono K, Tagiri A, Matsumoto T, Utsugi S, Ogawa T, Handa H, Ishida H, Mori M, Kawaura K, Ogihara Y, Miura H (2011) A Wheat Homolog of MOTHER OF FT AND TFL1 Acts in the Regulation of Germination. Plant Cell 23:3215–3229 - DOI - PubMed - PMC

[9] Pellegrineschi A, Noguera LM, Skovmand B, Brito RM, Velazquez L, Salgado MM, Hrnandez R, Warburton M, Hoisington D (2002) Identification of highly transformable wheat genotypes for mass production of fertile transgenic plants. Genome 45:421–430 - DOI - PubMed

[10] Pellegrineschi A, Noguera LM, Skovmand B, Brito RM, Velazquez L, Salgado MM, Hrnandez R, Warburton M, Hoisington D (2002) Identification of highly transformable wheat genotypes for mass production of fertile transgenic plants. Genome 45:421–430 - DOI - PubMed

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Functional Analysis of Seed Dormancy Genes by Biolistic Transient Gene Expression in Immature Embryos of Wheat

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Functional Analysis of Seed Dormancy Genes by Biolistic Transient Gene Expression in Immature Embryos of Wheat

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