Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Oct;598(19):2417-2437.
doi: 10.1002/1873-3468.14946. Epub 2024 Jun 10.

Creation of a novel CRISPR-generated allele to express HA epitope-tagged C1QL1 and improved methods for its detection at synapses

Hiu W Cheung  1 , Alexander D Schouw  1 , Zeynep M Altunay  1 , J Wesley Maddox  2 , Lyndsay C Kresic  1 , Brenna C McAllister  1 , Keaven Caro  1 , Shahnawaz Alam  1 , Angie Huang  2 , Robert S Pijewski  1   3 , Amy Lee  2 , David C Martinelli  1   4
Affiliations

Creation of a novel CRISPR-generated allele to express HA epitope-tagged C1QL1 and improved methods for its detection at synapses

Hiu W Cheung et al. FEBS Lett. 2024 Oct.

Abstract

C1QL1 is expressed in a subset of cells in the brain and likely has pleiotropic functions, including the regulation of neuron-to-neuron synapses. Research progress on C1QL proteins has been slowed by a dearth of available antibodies. Therefore, we created a novel knock-in mouse line in which an HA-tag is inserted into the endogenous C1ql1 locus. We examined the entire brain, identifying previously unappreciated nuclei expressing C1QL1, presumably in neurons. By total numbers, however, the large majority of C1QL1-expressing cells are of the oligodendrocyte lineage. Subcellular immunolocalization of synaptic cleft proteins is challenging, so we developed a new protocol to improve signal at synapses. Lastly, we compared various anti-HA antibodies to assist future investigations using this and likely other HA epitope-tagged alleles.

Keywords: ADGRB3; BAI3; CRISPR/Cas9; Ctrp14; oligodendrocyte; synapse.

PubMed Disclaimer

Conflict of interest statement

CONFLICT OF INTEREST STATEMENT

The authors declare no competing financial interests.

Similar articles

See all similar articles

References

    1. Pena Palomino PA, Black KC, and Ressl S, Pleiotropy of C1QL proteins across physiological systems and their emerging role in synapse homeostasis. Biochem Soc Trans, 2023. 51(3): p. 937–947. - PubMed
    1. Carland TM and Gerwick L, The C1q domain containing proteins: Where do they come from and what do they do? Dev Comp Immunol, 2010. 34(8): p. 785–90. - PubMed
    1. Yuzaki M, The C1q complement family of synaptic organizers: not just complementary. Curr Opin Neurobiol, 2017. 45: p. 9–15. - PubMed
    1. Sudhof TC, Cerebellin-neurexin complexes instructing synapse properties. Curr Opin Neurobiol, 2023. 81: p. 102727. - PubMed
    1. Ghai R, et al., C1q and its growing family. Immunobiology, 2007. 212(4–5): p. 253–66. - PubMed

LinkOut - more resources