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. 2024 Sep;79(3):571-577.
doi: 10.1007/s11130-024-01186-7. Epub 2024 May 25.

Mexican Coccoloba uvifera L. Leaf and Fruit Extracts: Identification of Pentacyclic Triterpenes and Volatile Profile by GC-MS

Montserrat Calderón-Santoyo  1 , Carolina Calderón-Chiu  1 , Frida Zoé Ragazzo-Calderón  1 , Julio César Barros-Castillo  1 , Juan Arturo Ragazzo-Sánchez  2
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Mexican Coccoloba uvifera L. Leaf and Fruit Extracts: Identification of Pentacyclic Triterpenes and Volatile Profile by GC-MS

Montserrat Calderón-Santoyo et al. Plant Foods Hum Nutr. 2024 Sep.

Abstract

Mexican Coccoloba uvifera fruit contains polyphenols, flavonoids, and anthocyanins, while in the leaves, lupeol, α- and β-amyrin have been previously identified by HPLC. However, the low resolution by HPLC of pentacyclic triterpenes (PTs) is a limitation. Moreover, the volatile profile of C. uvifera fruit is still unknown. Therefore, this study aimed to identify PTs in C. uvifera leaf and fruit extracts by CG-MS analysis and to determine the volatile profile of C. uvifera pulp by headspace solid-phase microextraction. The results showed trimethylsilylated compounds of standards lupeol, α- and β-amyrin, indicating that the silylation reaction was suitable. These trimethylsilylated compounds were identified in leaf and fruit extracts. The fruit volatile profile revealed the presence of 278 esters, 20 terpenes, 9 aldehydes, 5 alcohols, and 4 ketones. The fruit showed a high content of esters and terpenes. Due to their flavour properties, esters are essential for the food, cosmetics, and pharmaceutics industries. Moreover, terpenes in the fruit, such as menthone, β-elemene, junipene, and β-caryophyllene have the potential as anticancer and phytopathogen agents. The results indicated that GC-MS is an alternative to HPLC approaches for identifying PTs. Besides, identifying volatile compounds in the fruit will increase the value of this plant and expand its application. Identifying PTs and volatile compounds in Mexican C. uvifera leads to a better understanding of the potential benefits of this plant. This would increase the consumption of Mexican C. uvifera fresh or as functional ingredients in nutraceutical or pharmaceutical products.

Keywords: Coccoloba uvifera L; GC-MS; Pentacyclic Triterpenes; Silylation Trimethylsilylated; Volatile Profile.

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References

    1. Pinto T, Aires A, Cosme F et al (2021) Bioactive (poly)phenols, volatile compounds from vegetables, medicinal and aromatic plants. Foods 10:106. https://doi.org/10.3390/foods10010106 - DOI - PubMed - PMC
    1. Nisa S, Bibi Y, Waheed A et al (2011) Evaluation of anticancer activity of Debregeasia salicifolia extract against estrogen receptor positive cell line. Afr J Biotechnol 10:990–995. https://doi.org/10.5897/AJB10.696 - DOI
    1. Sasidharan S, Chen Y, Saravanan D et al (2010) Extraction, isolation and scharacterisation of bioactive compounds from plants’ extracts. Afr J Tradit Complement Altern Med. https://doi.org/10.4314/ajtcam.v8i1.60483 . 8: - DOI - PubMed - PMC
    1. James J, Dubery I (2009) Pentacyclic triterpenoids from the medicinal herb, Centella asiatica (L.) urban. Molecules 14:3922–3941. https://doi.org/10.3390/molecules14103922 - DOI - PubMed - PMC
    1. Abu El Wafa SA, Seif-Eldein A, Anwar Aly Taie N, Marzouk H M (2023) Coccoloba uvifera leaves: polyphenolic profile, cytotoxicity, and antioxidant evaluation. ACS Omega 8:32060–32066. https://doi.org/10.1021/acsomega.3c04025 - DOI - PubMed - PMC

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