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. 2024 Sep 6;24(5):1170-1177.
doi: 10.17305/bb.2024.10237.

The relationship of peripheral blood lncRNA-PVT1 and miR-146a levels with Th17/Treg cytokines in patients with Hashimoto's thyroiditis and their clinical significance

Yi-Nan Li  1 , Jingxue Shen  1 , Yinglan Feng  2 , Yingyan Zhang  1 , Yusi Wang  1 , Xinyu Ren  1
Affiliations

The relationship of peripheral blood lncRNA-PVT1 and miR-146a levels with Th17/Treg cytokines in patients with Hashimoto's thyroiditis and their clinical significance

Yi-Nan Li et al. Biomol Biomed. .

Abstract

Hashimoto's thyroiditis (HT) is a prevalent autoimmune disease. We investigated the relationship of peripheral blood long noncoding RNA-plasmacytoma variant translocation 1 (lncRNA-PVT1) and microRNA (miR)-146a levels with Th17/Treg-related cytokines in HT patients and their clinical significance. Correlations of PVT1 and miR-146a with Th17/Treg-related cytokines were analyzed, and its clinical value in diagnosing HT was assessed. Results showed reduced lncRNA-PVT1 and interleukin (IL)-10 levels and increased miR-146a and IL-17 levels in HT patients. lncRNA-PVT1 negatively interrelated with miR-146a, IL-17, IL-23 and IL-6, and positively interrelated with IL-10; miR-146a positively correlated with IL-17, IL-23 and IL-6, but negatively correlated with IL-10 in HT patients. The area under the curve (AUC) of lncRNA-PVT1 and miR-146a levels for diagnosing HT were 0.822 and 0.844, respectively (sensitivity 88.73% and 86.62%, specificity 67.02% and 69.15%, cut-off values 0.76 and 2.73), with their combined detections yielding a higher AUC. Patients with poorly expressed lncRNA-PVT1 and highly expressed miR-146a had elevated HT incidence. lncRNA-PVT1 and miR-146a levels were also found to be an independent influencing factor for HT occurrence. Our findings suggest that HT patients have low peripheral blood lncRNA-PVT1 expression and high miR-146a expression. lncRNA-PVT1 and miR-146a level changes were correlated with Th17/Treg cytokine imbalance and could be a potential diagnostic tool and independent influencing factor for HT.

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Conflict of interest statement

Conflicts of interest: Authors declare no conflicts of interest.

Figures

Figure 1.
Figure 1.
Comparisons of peripheral blood lncRNA-PVT1 and miR-146a expression levels in HT patients. (A and B) RT-qPCR to measure peripheral blood lncRNA-PVT1 and miR-146a expression patterns of the HT and NC groups; (C) Pearson correlation coefficient to analyze the correlations between peripheral blood lncRNA-PVT1 and miR-146a of HT patients. Data are expressed as mean ± SD. Comparisons between the two groups were performed by t-test. *P < 0.05, **P < 0.01. HT: Hashimoto’s thyroiditis; SD: Standard deviation; NC: Normal control group; RT-qPCR: Reverse transcription-quantitative polymerase chain reaction.
Figure 2.
Figure 2.
Comparisons of peripheral blood Th17/Treg-related cytokine levels in HT patients. (A–D) Th17/Treg-related cytokine (IL-17, IL-23, IL-6, IL-10) levels in the HT and NC groups were measured by ELISA. Comparisons of data between groups: *P < 0.05, **P < 0.01. HT: Hashimoto’s thyroiditis; NC: Normal control group; ELISA: Enzyme-linked immunosorbent assay.
Figure 3.
Figure 3.
ROC curve analysis of the value of lncRNA-PVT1, miR-146a alone and their combination for HT diagnosis. ROC: Receiver operating characteristic; HT: Hashimoto’s thyroiditis.
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