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. 2024 May 9;22(1):217.
doi: 10.1186/s12951-024-02490-9.

Molybdesum selenide-based platelet-rich plasma containing carboxymethyl chitosan/polyvinyl pyrrolidone composite antioxidant hydrogels dressing promotes the wound healing

Xiaoyi Zheng #  1 , Yongliang Ouyang #  2 , Hengwei Fan #  3 , Liying Zhang  2 , Shige Wang  2 , Yanbo Zeng  4 , Lianghao Hu  5 , Jiulong Zhao  6
Affiliations

Molybdesum selenide-based platelet-rich plasma containing carboxymethyl chitosan/polyvinyl pyrrolidone composite antioxidant hydrogels dressing promotes the wound healing

Xiaoyi Zheng et al. J Nanobiotechnology. .

Abstract

Excess free radicals at the wound site can cause an inflammatory response, which is not conducive to wound healing. Hydrogels with antioxidant properties can prevent inflammatory storms by scavenging free radicals from the wound site and inhibiting the release of inflammatory factors. In this study, we prepared the carboxymethyl chitosan (CMCS)/polyvinyl pyrrolidone (PVP)/Molybdenum (IV) Selenide (MoSe2), and platelet-rich plasma (PRP) (CMCS/PVP/MoSe2/PRP) hydrogels for accelerating the repair of wounds. In the hydrogels, the MoSe2 can scavenge various free radicals to reduce oxidative stress at the site of inflammation, endowed the hydrogels with antioxidant properties. Interestingly, growth factors released by PRP assisted the tissue repair by promoting the formation of new capillaries. CMCS as a backbone not only showed good biocompatibility and biodegradability but also played a significant role in maintaining the sustained release of growth factors. In addition, incorporating PVP enhanced the tissue adhesion and mechanical properties. The multifunctional composite antioxidant hydrogels have good swelling properties and biodegradability, which is completely degraded within 28 days. Thus, the antioxidant CMCS/PVP/MoSe2/PRP hydrogels provide a new idea for designing ideal multifunctional wound dressings.

Keywords: Hydrogels; Molybdenum selenide; Oxygen radicals; Wound dressing.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Scheme 1
Scheme 1
Schematic representation of the multifunctional CMCS/PVP/MoSe2/PRP hydrogels for scavenging free radicals from the wound site and releasing multiple factors to promote wound healing (Created by Biorender.com)
Fig. 1
Fig. 1
(a) Digital images of the hydrogels formation process of CMCS/PVP/MoSe2/PRP hydrogels; (b)—(d) SEM images of CMCS/PVP hydrogels ((b): CMCS/PVP (1); (c) CMCS/PVP (2); (d) CMCS/PVP (3); (e)) swelling ratio of CMCS/PVP hydrogels in PBS
Fig. 2
Fig. 2
(a) SEM image of CMCS/PVP/MoSe2 hydrogels; (b) SEM image of CMCS/PVP/MoSe2/PRP hydrogels; (c) digital image of the prepared hydrogels (from left to right: CMCS/PVP hydrogels, CMCS/PVP/MoSe2 hydrogels, and CMCS/PVP/MoSe2/PRP hydrogels); (d)—(f) distribution of O, Se and Mo elements in CMCS/PVP/MoSe2/PRP hydrogels
Fig. 3
Fig. 3
(a) FTIR spectra of PVP, CMCS, and CMCS/PVP/MoSe2/PRP hydrogels; (b) XPS spectrum of Mo 3d; (c) XPS spectrum of Se 3d; (d) and (e) swelling curves of hydrogels in (d) PBS and (e) H2O; (f)in vitro degradation rate curves of CMCS/PVP/MoSe2/PRP hydrogels in PBS solution and H2O; (g) strain-stress test picture (left: before stretching, right: after stretching); (h) strain-stress curves for shear tests and (i) final average tensile strength; (j) macroscopic images of CMCS/PVP/MoSe2 hydrogels in compression (top: before compression, bottom: after compression) (j) strain-stress curves for compression tests and (k) final mean compressive strength of hydrogels
Fig. 4
Fig. 4
(a) UV-Vis absorption spectra of salicylic acid without (control) and with CMCS/PVP/MoSe2/PRP hydrogels; (b) digital photographs of the co-incubated solutions corresponding to (a); (c) scavenging ratio of ·OH radicals by CMCS/PVP/MoSe2/PRP hydrogels; (d) ESR spectra of DMPO/O2·− after incubated with CMCS/PVP/MoSe2/PRP hydrogels
Fig. 5
Fig. 5
(a) UV-Vis spectra of the DPPH solution after co-incubation of CMCS/PVP/MoSe2/PRP hydrogels with different MoSe2 concentrations; (b) digital images of the solution corresponding to (a); (c) UV-Vis spectra of the solution after co-incubation of TMB with CMCS/PVP/MoSe2/PRP hydrogels in the presence of H2O2; (d) digital images of the solution corresponding to (c); (e) UV-Vis spectra of CMCS/PVP/MoSe2/PRP hydrogels solution after co-incubation with PTIO; (f) digital images of the solutions corresponding to (e); (g) and (h) release profile of (g) VEGF and (h) EGF from CMCS/PVP/MoSe2/PRP hydrogels
Fig. 6
Fig. 6
(a) Images of L929 cell migration after co-cultured with CMCS/PVP/MoSe2/PRP hydrogels; (b) cell migration ratio at different time points; (c) hemolysis ratio of erythrocytes after treatment with CMCS/PVP/MoSe2/PRP hydrogels and digital images of the corresponding solutions; (d) CCK-8 results of L929 cells treated with CMCS/PVP/MoSe2/PRP hydrogels extracts; (e) images of live/dead cell staining of L929 cells after treatment with CMCS/PVP/MoSe2/PRP hydrogels extracts
Fig. 7
Fig. 7
(a) Body weight change curves of KM mice after different treatments; (b)in vivo degradation curves of CMCS/PVP/MoSe2/PRP hydrogels and corresponding digital images; (c) blood biochemical results of KM mice after hydrogels treatment at different time points; (d) flow chart of the trauma wound healing experiments (created by Biorender.com); (e) representative photographs of wound healing in Balb/c mice treated with different hydrogels on days 0, 2, 5, 8, 12, and 16
Fig. 8
Fig. 8
(a) Wound healing ratio in Balb/c mice treated with different hydrogels on days 0, 2, 5, 8, 12, and 16; (b) levels of EGF, VEGF, and TNF-α in serum of mice treated with different hydrogels (day 16); (c) levels of EGF, VEGF, and TNF-α in mouse tissues treated with different hydrogels (day 8 and day 16). (d) representative images of H&E staining, Masson staining, and Sirius Red staining of the healing skin of Balb/c mice obtained on day 16 (Bar = 200 μm); (e) representative images of CD31 staining of healing skin of Balb/c mice obtained on day 16 (Bar = 200 μm); (f) representative images of α-SMA staining of healing skin of Balb/c mice obtained on day 16 (Bar = 200 μm)
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