A novel approach in biomedical engineering: The use of polyvinyl alcohol hydrogel encapsulating human umbilical cord mesenchymal stem cell-derived exosomes for enhanced osteogenic differentiation and angiogenesis in bone regeneration
- PMID: 38723803
- DOI: 10.1016/j.ijbiomac.2024.132116
A novel approach in biomedical engineering: The use of polyvinyl alcohol hydrogel encapsulating human umbilical cord mesenchymal stem cell-derived exosomes for enhanced osteogenic differentiation and angiogenesis in bone regeneration
Abstract
Developing effective methods for alveolar bone defect regeneration is a significant challenge in orthopedics. Exosomes from human umbilical cord mesenchymal stem cells (HUMSC-Exos) have shown potential in bone repair but face limitations due to undefined application methods and mechanisms. To address this, HUMSC-Exos were encapsulated in polyvinyl alcohol (PVA) hydrogel (Exo@PVA) to create a novel material for alveolar bone repair. This combination enhanced the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and human umbilical vein endothelial cells (HUVECs) more effectively than Exos alone. Additionally, Exo@PVA significantly improved alveolar bone regeneration and defect repair in rats. The microRNA-21-5p (miR-21-5p) in Exo@PVA, identified through the GEO database and analyzed via in silico methods, played a crucial role. miR-21-5p promoted BMSC osteogenic differentiation by inhibiting WWP1-mediated KLF5 ubiquitination and enhanced HUVEC angiogenesis by targeting ATP2B4. These findings underscore the potential of an Exo-based approach with PVA hydrogel scaffolds for bone defect repair, operating through the miR-21-5p/WWP1/ATP2B4 signaling axis.
Keywords: ATP2B4; Alveolar bone defect; Exosomes; Human umbilical cord mesenchymal stem cells; KLF5 ubiquitination; Polyvinyl alcohol hydrogel; WWP1; miR-21-5p.
Copyright © 2024. Published by Elsevier B.V.
Conflict of interest statement
Declaration of competing interest The authors declare no competing financial interest.
Similar articles
-
Umbilical Mesenchymal Stem Cell-Derived Exosome-Encapsulated Hydrogels Accelerate Bone Repair by Enhancing Angiogenesis.ACS Appl Mater Interfaces. 2021 Apr 28;13(16):18472-18487. doi: 10.1021/acsami.0c22671. Epub 2021 Apr 15. ACS Appl Mater Interfaces. 2021. PMID: 33856781
-
Enhancing bone regeneration and immunomodulation via gelatin methacryloyl hydrogel-encapsulated exosomes from osteogenic pre-differentiated mesenchymal stem cells.J Colloid Interface Sci. 2024 Oct 15;672:179-199. doi: 10.1016/j.jcis.2024.05.209. Epub 2024 May 29. J Colloid Interface Sci. 2024. PMID: 38838627
-
Impaired Bone Regenerative Effect of Exosomes Derived from Bone Marrow Mesenchymal Stem Cells in Type 1 Diabetes.Stem Cells Transl Med. 2019 Jun;8(6):593-605. doi: 10.1002/sctm.18-0199. Epub 2019 Feb 26. Stem Cells Transl Med. 2019. PMID: 30806487 Free PMC article.
-
Extracellular vesicle-loaded hydrogels for tissue repair and regeneration.Mater Today Bio. 2022 Dec 21;18:100522. doi: 10.1016/j.mtbio.2022.100522. eCollection 2023 Feb. Mater Today Bio. 2022. PMID: 36593913 Free PMC article. Review.
-
The potential therapeutic effect of human umbilical cord mesenchymal stem cell-derived exosomes in bronchopulmonary dysplasia.Life Sci. 2024 Nov 15;357:123047. doi: 10.1016/j.lfs.2024.123047. Epub 2024 Sep 12. Life Sci. 2024. PMID: 39260518 Review.
Cited by
-
Exosomal miRNA-21 derived from umbilical cord mesenchymal stem cells inhibits microglial overactivation to counteract nerve damage.Mol Biol Rep. 2024 Aug 28;51(1):941. doi: 10.1007/s11033-024-09878-8. Mol Biol Rep. 2024. PMID: 39196412
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
