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. 2024 Mar 21;40(1):87.
doi: 10.1007/s00383-024-05667-3.

Children with Hirschsprung disease exhibited alterations in host-microbial co-metabolism after pull-through operation

Kanokrat Thaiwatcharamas  1 , Watcharin Loilome  2   3   4 , Phuc N Ho  2 , Sinobol Chusilp  1 , Patchareeporn Tanming  1 , Poramate Klanrit  2   3   4 , Jutarop Phetcharaburanin  5   6   7
Affiliations

Children with Hirschsprung disease exhibited alterations in host-microbial co-metabolism after pull-through operation

Kanokrat Thaiwatcharamas et al. Pediatr Surg Int. .

Abstract

Purpose: This study aims to compare the fecal metabolome in post pull-through HD with and without HAEC patients and healthy young children using nuclear magnetic resonance (NMR) spectroscopy.

Methods: Fresh fecal samples were collected from children under 5 years of age in both post-pull-through HD patients and healthy Thai children. A total of 20 fecal samples were then analyzed using NMR spectroscopy.

Results: Thirty-four metabolites identified among HD and healthy children younger than 5 years were compared. HD samples demonstrated a significant decrease in acetoin, phenylacetylglutamine, and N-acetylornithine (corrected p value = 0.01, 0.04, and 0.004, respectively). Succinate and xylose significantly decreased in HD with HAEC group compared to HD without HAEC group (corrected p value = 0.04 and 0.02, respectively). Moreover, glutamine and glutamate metabolism, and alanine, aspartate, and glutamate metabolism were the significant pathways involved, with pathway impact 0.42 and 0.50, respectively (corrected p value = 0.02 and 0.04, respectively).

Conclusion: Differences in class, quantity, and metabolism of protein and other metabolites in young children with HD after pull-through operation were identified. Most of the associated metabolic pathways were correlated with the amino acids metabolism, which is required to maintain intestinal integrity and function.

Keywords: Hirschsprung disease; Hirschsprung-associated enterocolitis; Metabolome; Nuclear magnetic resonance spectroscopy.

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