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Review
. 2024 Feb 1;84(3):415-428.
doi: 10.1016/j.molcel.2023.12.032. Epub 2024 Jan 18.

Epigenetic regulatory layers in the 3D nucleus

Andréa Willemin  1 , Dominik Szabó  2 , Ana Pombo  3
Affiliations
Review

Epigenetic regulatory layers in the 3D nucleus

Andréa Willemin et al. Mol Cell. .

Abstract

Nearly 7 decades have elapsed since Francis Crick introduced the central dogma of molecular biology, as part of his ideas on protein synthesis, setting the fundamental rules of sequence information transfer from DNA to RNAs and proteins. We have since learned that gene expression is finely tuned in time and space, due to the activities of RNAs and proteins on regulatory DNA elements, and through cell-type-specific three-dimensional conformations of the genome. Here, we review major advances in genome biology and discuss a set of ideas on gene regulation and highlight how various biomolecular assemblies lead to the formation of structural and regulatory features within the nucleus, with roles in transcriptional control. We conclude by suggesting further developments that will help capture the complex, dynamic, and often spatially restricted events that govern gene expression in mammalian cells.

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Conflict of interest statement

Declaration of interests A.P. holds a patent on “Genome Architecture Mapping”: Pombo, A., Edwards, P.A.W., Nicodemi, M., Scialdone, A., and Beagrie, R.A. Patent PCT/EP2015/079413 (2015).

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